|
| 耐力运动对骨质疏松小鼠骨量、骨形态学及Linc-ROR表达的影响 |
| The effect of endurance exercise on bone loss, morphological deterioration, and expression of Linc-ROR in ovariectomized mice |
| |
| DOI：10.3969/j.issn.1006-7108.2021.07.008 |
| 中文关键词: 骨质疏松 耐力运动 长链非编码 RNA Wnt/-catenin |
| 英文关键词:osteoporosis endurance training Lnc-RNA Wnt/-catenin |
| 基金项目:江苏省自然科学基金（BK20201435） |
|
| 摘要点击次数: 420 |
| 全文下载次数: 272 |
| 中文摘要: |
| 目的 研究长链非编码 RNA?-linc-ROR在运动预防卵巢切除小鼠骨量丢失、改善骨形态学破坏中的作用。方法 24只3月龄雌性 C57BL小鼠随机分为假手术组（Sham）、卵巢切除组（Ovx）、卵巢切运动组（Ex）。Ex小鼠接受8周下坡跑训练，8周后所有小鼠处死，收集血液、骨骼标本检测血清激素（ELISA）、骨密度（bone mineral density，BMD）、骨形态学指标（micro-CT）及相关基因（PCR）与蛋白（Western blot）表达。采用STATA 15 软件统计组间差异。结果 Ovx小鼠出现BMD下降，骨体积(bone volume/total volume，BV/TV)、骨小梁厚度(trabecular thickness，Tb.Th)、骨小梁数量(trabecular number，Tb.N)减小，骨小梁间距(trabecular separation，Tb.Sp)增加；运动增加Ovx小鼠BMD、BV/TV、Tb.Th和Tb.N，减少Tb.Sp。相比Ovx组，Ex小鼠骨组织中碱性磷酸酶阳性（alkaline phosphatase positive，ALP+）成骨细胞数量增多。Ovx小鼠血清雌二醇（estradiol，E2）、骨保护素(osteoprotegerin，OPG)下降，核因子κB受体配体（receptor activator of NF-κB ligand，RANKL）升高，运动明显增加血清E2、OPG水平，降低RANKL浓度。Ovx 小鼠骨组织 Linc-ROR及 Wnt3、?-catenin mRNA和蛋白表达下降，运动上调这些基因、蛋白的表达。结论 耐力运动可预防骨质疏松小鼠骨量丢失、改善骨微观结构，Linc-ROR/Wnt/?-catenin 信号通路可能参与这一过程。 |
| 英文摘要: |
| Objective To determine the role of Linc-ROR in regulating the effect of exercise on bone loss in ovariectomized (Ovx) mice. Methods Twenty-four 3-month C57BL mice were randomly divided into sham, Ovx, and exercise (Ex) group. Ex mice received 8-week running at the speed of 0.8 km per hour for 5 days per week. After finishing the training, all mice were killed and their blood and bone samples were collected. Serum hormone levels, bone mineral density (BMD), bone histomorphometry parameters, the relative genes and proteins were determined. The differences among the groups was analyzed using a STATA15 software. Results Ovx mice showed reduced vBMD, bone volume/total volume (BV/TV), trabecular thickness (Tb.Th) and trabecular number (Tb.N), and enlarged trabecular separation (Tb.Sp). Exercise promoted BMD, BV/TV, Tb.Th, and Tb.N, and reduced Tb.Sp in Ovx mice. Exercise also increased alkaline phosphatase positive (ALP+) osteoblasts in bone tissue in Ovx mice. Ovx mice had decreased serum estradiol (E2) and osteoprotegerin (OPG) and elevated receptor activator of NF-κB ligand (RANKL) levels. Exercise significantly improved E2 and OPG, and reduced RANKL. Ovx mice showed a decreased expressions of Linc-ROR, Wnt3, and ?-catenin mRNA and protein. Exercise elevated those gene and protein expressions. Conclusion Endurance exercise prevents bone loss and impaired bone microarchitecture from osteoporosis. Linc-ROR/Wnt/?-catenin pathway may participate in this process. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|