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| 芪骨胶囊治疗绝经后骨质疏松症的分子机制 |
| Study on the mechanism of Qigu capsules in the treating postmenopausal osteoporosis |
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| DOI：10.3969/j.issn.1006-7108.2021.08.001 |
| 中文关键词: 芪骨胶囊 绝经后骨质疏松症 网络药理学 |
| 英文关键词:Qigu capsules postmenopausal osteoporosis network pharmacology |
| 基金项目:国家自然科学基金（81674003；81774342）；上海市进一步加快中医药事业发展三年行动计划（ZY（2018-2020）-CCCX-2003-05）；上海市浦东新区卫生系统重点学科群“老年骨骼疾病防治”（PWZxq2014-10）；上海中医药大学预算内项目（2020LK046） |
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| 中文摘要: |
| 目的 运用网络药理学的方法探究芪骨胶囊治疗绝经后骨质疏松症（postmenopausal osteoporosis，PMOP）的活性成分与潜在分子机制。方法 从BATMAN-TCM数据库中获取芪骨胶囊的主要活性成分及其对应的靶点，在GeneCards数据库和GEO数据库中筛选PMOP疾病靶点，将药物靶点与疾病靶点取交集，运用Cytoscape软件构建“药物-成分-靶点”网络图，同时将交集靶点导入String在线软件构建蛋白互作网络，筛选出核心靶点进行GO（gene ontology）生物过程及KEGG（Kyoto encyclopedia of genes and genomes）通路富集分析。结果 筛选获得67个活性成分，核心活性成分包括24-羟基-11-脱氧甘草次酸、菊油环酮、β-谷甾醇、γ-谷甾醇等；获得交集靶点共57个，其中核心靶点有胰岛素（INS）、白介素-6（IL-6）、胰岛素样生长因子-1（IGF-1）等。核心靶点GO生物过程和KEGG通路富集分析结果显示，GO生物过程主要涉及女性怀孕、多细胞生物过程等，KEGG通路主要涉及雌激素信号通路、卵巢甾体生成、流体剪切应力与动脉粥样硬化、白细胞介素17信号通路等。结论 对于芪骨胶囊治疗PMOP的作用机制可能与菊花、黄芪、肉苁蓉中的主要成分如α-蒎烯、菊油环酮、β-谷甾醇等有关，并可能通过雌激素、白细胞介素等信号通路作用于人体INS、IL-6、IGF-1等靶点发挥疗效。 |
| 英文摘要: |
| Objective To explore the active components and potential molecular mechanism of Qigu capsule in the treatment of postmenopausal osteoporosis (postmenopausal osteoporosis, PMOP) by means of network pharmacology. Methods The main active components and corresponding targets of Qigu capsule were obtained from BATMAN-TCM database. The disease targets of PMOP were screened in GeneCards database and GEO database. The drug targets and disease targets were intersected. The drug-component-target network was constructed by using Cytoscape software. At the same time, the intersection targets were introduced into String online software to construct protein interaction network. The core targets were selected for GO (gene ontology) biological process and KEGG (Kyoto encyclopedia of genes and genomes) pathway enrichment analysis. Results 67 active components were obtained, including 24-hydroxy-11-deoxyglycyrrhetinic acid, chrysanthemum oil cyclone, β-sitosterol, γ-sitosterol and so on. A total of 57 intersection targets were obtained, including insulin (INS), interleukin-6 (IL-6), insulin-like growth factor-1 (IGF-1) and so on. The results of core target GO biological process and KEGG pathway enrichment analysis show that GO biological process is mainly involved in female pregnancy, multicellular biological process and so on. KEGG pathway is mainly involved in estrogen signal pathway, ovarian steroid production, fluid shear stress and atherosclerosis, interleukin-17 signal pathway and so on. Conclusion The mechanism of Qigu capsule in treating PMOP may be related to the main components of chrysanthemum, astragalus and Cistanche deserticola, such as α-pinene, chrysanthemum oil cyclone, β-sitosterol, etc., and may act on human INS, IL-6, IGF-1 and other targets through estrogen and interleukin signal pathways. |
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