| Objective Based on network pharmacology and molecular docking methods, the effective ingredients and mechanism of Liuwei Dihuang Pills in the treatment of osteoporosis were discussed. Method Obtain the active ingredients of Liuwei Dihuang Pills and their predicted targets through the TCMSP database; Use the disease database to screen the targets of osteoporosis-related diseases; Upload the obtained key targets to the STRING database to complete the PPI network analysis; Use DAVID database to complete the enrichment analysis of key targets; Use Auto Dock Vina and other software to complete the analysis and docking verification of chemically effective pharmaceutical ingredients and key targets. Results A total of 69 chemical active ingredients in Liuwei Dihuang Pill formula, 128 drug target proteins, and 81 drug and osteoporosis common related targets were obtained. Key targets in the PPI network such as IL6, TP53, VEGFA, TNF, JUN, MAPK1, EGF, EGFR, ESR1, CAT, etc. have been obtained. Enrichment analysis results show that it regulates TNF signaling pathway, drug metabolism process signaling pathway, and HIF-1 signaling by participating in biological processes such as cell proliferation and apoptosis, drug metabolism, estrogen response regulation, and cell anti-anoxia and anti-aging. Pathways, VEGF signaling pathways, estrogen regulatory signaling pathways and other signaling pathways, which are involved in the treatment of osteoporosis. The results of molecular docking show that the key targets in the regulatory network have high binding activity with relevant active ingredients. Conclusion With the help of bioinformatics methods and molecular docking technology, the anti-osteoporotic mechanism of Liuwei Dihuang Pills is initially revealed, which provides goals and ideas for subsequent further mechanism research and experimental verification. |