| Objective To compare the inhibitory effects of zoledronate, ibandronate and pamidronate on the function of osteoclasts, and draw characteristic curves to guide clinical application. Methods Osteoclasts were obtained by culturing mouse bone marrow cells in vitro and divided into zoledronate group, ibandronate group, pamidronate group and control group. Different concentrations of zoledronate were added to the medium of zoledronate group, and the gradient of concentrations were 10-3，10-2，10-1，1，10，102，103 μmol/L. The same concentrations of ibandronate and pamidronate was added to ibandronate group and pamidronate group. Phosphate was used as control. The inhibiting effects of osteoclasts formation, migration, adhesion and bone resorption were observed and compared. Results It was found that the number of polynuclear cells decreased with the increase of concentration. The lowest effective inhibitory concentrations of zoledronate, ibandronate and pamidronate were 1,10,102 μmol/L. The 50% effective concentrations of three drugs were 0.66,5.58,51.9 μmol/L. Conclusion It is suggested that the clinical treatment of osteoporosis should refer to the inhibition characteristic curve of osteoclasts, to provide scientific basis for intravenous application of zoledronate, ibandronate and pamidronate. |