| Objective To investigate the mechanism of Jiangu Granule in the treatment of postmenopausal osteoporosis by metabonomics. Methods Sixty rats were randomly divided into normal group, model group and Jiangu Granule group.The Jiangu granule group was given Jiangu granule solution by gavage, and the others were given the same capacity saline by gavage. After 6 week or 12 week, the femur, tibia and lumbar vertebrae were used for the test of bone mineral density(BMD) and biomechanics. The blood of abdominal aorta was analyzed by liquid chromatography-mass spectrometry (LC-MS). After univariate statistics and multivariate statistics analysis, the potential metabolites were selected. Results Compared with the normal group, the BMD and maximum load of model group decreased significantly, while the Jiangu Granule group tended to return to normal level. It was indicated that the model was successful and the rat body was tended to return to normal. The serum metabolic spectrum of the model group and the normal group were completely separated. Jiangu Granule group was close to the normal group. 27 different metabolites, such as pantothenate, stearic acid and corticosterone, were screened out, which were related to the biosynthesis of pantothenate and CoA , unsaturated fatty acids and steroid hormones, respectively. These metabolites were involved in lipid metabolism, nucleic acid metabolism, amino acid metabolism and so on. Conclusion Jiangu granule may treat postmenopausal osteoporosis by regulated multiple metabolic pathways. |