| Objective To explore the active ingredients, targets and mechanisms of Osteoking(OK, Herbs of the Yi Minority in Yunnan Province) in the treatment of osteoporosis (OP) with the help of systematic pharmacology. And the molecular docking technology is used for docking verification. Methods Screened the active components and targets of OK through TCMSP. Queried GeneCard, OMIM, DisGeNet, TTD Databases to Screening of OP-related gene targets. Constructed the intersection gene target protein-protein interaction(PPI) network by using STRING and Cytoscape Software.The core targets were analyzed by GO and KEGG with the help of DAVID and KEGG Mapper database.Finally, Autodock was used for docking verification. Results The main active components such as quercetin, kaempferoland,11-octadecenoic acid,11-eicosenoic acid,ursolic acid,beta-sitosterol,tormentic acid were collected. It involves biological processes such as cell responses to lipids, toxic substances, lipopolysaccharides, bacterial molecules, inorganic substances, organic ring compounds, organic nitrogen compounds and so on.Theymight play rolesthrough PI3K-Akt, Ras, MAPK, ect multiple pathways.Molecular docking suggested that ursolic acid had better binding effect with AKT1, ALB and IL-6.Conclusion It is preliminarily clear that OK participates in promoting bone formation and inhibiting bone fracture through multi-targets and multi-pathways, which provides theoretical support and design ideas for further experimental research. |