| Objective To investigate the mechanism of of Jinkuishenqi pills (JSP) in the prevention and treatment of osteoporosis. Methods The active components and targets of JSP were screened from TCMSP database. The network map of herb-ingredient-target of JSP was constructed using Cytoscape 3.6. The disease genes of osteoporosis were searched in DisGeNET and GeneCards online databases. After the intersection of these genes with the target genes of JSP, PPI network information was obtained from STRING database. Cytoscape 3.6 was used to construct the network diagram. David was used to perform the GO biological function and KEGG pathway enrichment analysis on key genes. Omicshare was used to draw the advanced bubble map. The key pathways were found and key genes were labeled using KEGG database. Results There were 47 active components corresponding to 196 target genes, 145 intersecting target genes with osteoporosis disease genes, and 30 key genes were screened out from JSP. The results of GO enrichment analysis showed that the biological pathways mainly included response to drug, positive regulation of transcription, DNA templates, and positive regulation of transcription from RNA polymerase II promoter. The molecular functions mainly include enzyme binding, identical protein binding, and protein binding, etc. The cell components were divided into nucleoplasm, cytosol, and nucleus, etc. The key genes were mainly enriched in TNF, MAPK, PI3K-Akt, and other signaling pathways. Conclusion Quercetin, stigmasterol, beta-sitostero, kaempferol, and other core active ingredients in JSP intervene the expression of the key target genes AKT1, TP53, IL6, MAPK1, MAPK8, and JUN, through TNF, MAPK, PI3K-Akt, HIF-1 signaling pathways, promote osteoblast formation, inhibit osteoclast differentiation, and maintain the balance of bone metabolism, and achieve the purpose of prevention and treatment of osteoporosis. |