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| 芝麻素对大鼠骨质疏松症的作用研究 |
| Study on the effect of sesamin on osteoporosis in rats |
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| DOI：10.3969/j.issn.1006.7108.2022.03.024 |
| 中文关键词: 芝麻素 骨质疏松 去卵巢大鼠 骨钙素 I型胶原 |
| 英文关键词:sesamin osteoporosis ovariectomized rats osteocalcin type I collagen |
| 基金项目:内蒙古医科大学校基金 (YKD2015KJBW011) |
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| 中文摘要: |
| 目的 在大鼠模型中探讨芝麻素对骨质结构改变的作用及对骨钙素和I型胶原表达的影响。方法 建立去卵巢(ovariectomized, OVX)大鼠模型，给予芝麻素(sesamin, 80 mg/kg)灌胃作为干预措施并同时建立对照组。应用显微计算机断层扫描(Micro-CT)对大鼠股骨进行分析，并应用免疫组化及免疫荧光检测大鼠股骨中的骨钙素(osteocalcin, OCN)水平以及I型胶原表达。结果 本实验研究条件下，与OVX组相比，OVX+S骨密度(bone mineral density, BMD)增加、小梁间距(trabecular spacing, Tb.Sp)降低，同时，干预组骨钙素和I型胶原表达显著增强(P<0.05)。结论 芝麻素可有效促进去卵巢大鼠骨结构重塑，对大鼠骨质疏松症有积极的治疗和预防作用。 |
| 英文摘要: |
| Objective To investigate the effect of sesamin on the changes of bone structure and the expression of osteocalcin and type I collagen in the rat model. Methods Ovariectomized (OVX) rat model was established. Rats received sesamin (80 mg/kg) by intragastric administration as an intervention and a control group was established at the same time. The femur of rats was analyzed using micro-computed tomography (micro-CT). The content of osteocalcin (OCN) and the expression of type I collagen in the femur were detected with immunohistochemistry and immunofluorescence. Results Compared to those in the OVX group, bone mineral density (BMD) of OVX+S increased and the trabecular spacing (Tb.Sp) decreased in the experimental group. At the same time, the expressions of osteocalcin and type I collagen in the intervention group was significantly higher than those in the intervention group (P<0.05). Conclusion Sesamin effectively promotes the remodeling of bone structure in ovariectomized rats and has a positive effect on the treatment and prevention of osteoporosis in rats. |
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