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| 持续激活β连环蛋白对老年和成年小鼠骨量影响的研究 |
| Effects of continuous activation of β-catenin on bone mass in aged and adult mice |
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| DOI：10.3969/j.issn.1006.7108.2022.04.005 |
| 中文关键词: β-catenin 骨质疏松 成骨细胞 破骨细胞 |
| 英文关键词:β-catenin osteoprosis osteoblast osteoclaste |
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| 中文摘要: |
| 目的 探讨持续激活β连环蛋白（β-catenin）后对老年和成年小鼠骨量的影响。方法 取3.2Cre; Catnb+/lox(exon3)小鼠，月龄为4月小鼠6只，月龄18月小鼠6只，另取月龄相同的6只同窝wide type（WT）小鼠作为对照。持续激活β-catenin（注射他莫昔芬启动激活）4周后取小鼠胫骨标本，行胫骨干骺端CT扫描并分析数据，通过病理组织切片染色观察小鼠成骨及破骨细胞变化水平，血清学检测成骨（ALP）及破骨（CTCX1）指标。结果 3.2Cre; Catnb+/lox(exon3) 小鼠与同组WT小鼠对比胫骨骨量均增加（P<0.05）；病理组织染色提示各组中3.2Cre; Catnb+/lox(exon3)小鼠相比WT小鼠其胫骨处成骨细胞阳性数量均增加（P<0.05），破骨细胞少于WT小鼠；血清学实验结果提示Catnb+/lox(exon3)小鼠骨形成指标（ALP）均上升，骨吸收相关指标（CTCX1）均下降（P均<0.05）。CT扫描结果显示老年组3.2Cre; Catnb+/lox(exon3)小鼠骨量比成年组3.2Cre; Catnb+/lox(exon3)小鼠骨量仍少（P<0.05），但对比于成年Catnb+/lox(exon3)小鼠，持续激活β-catenin后骨量增加幅度大于成年3.2Cre; Catnb+/lox(exon3)小鼠（P<0.05）；破骨细胞染色结果提示老年组3.2Cre; Catnb+/lox(exon3)小鼠破骨细胞数量仍多于成年组3.2Cre; Catnb+/lox(exon3)小鼠（P<0.05）。结论 持续激活β连环蛋白后可使成年及老年3.2Cre; Catnb+/lox(exon3)小鼠骨量均增多，而老年组3.2Cre; Catnb+/lox(exon3)小鼠骨量增加幅度大于成年3.2Cre; Catnb+/lox(exon3)小鼠。 |
| 英文摘要: |
| Objective We want to know the effect of activating β-catenin on bone mass increase in aged and adult mice. Methods Take 4 months old and 18 months old 3.2Cre; Catnb+/lox(exon3) mice, and take an equal number of littermate wide type (WT) mice as controls. An intraperitoneal injection of Tamoxifen(TM) was used to continued activate β-catenin, and the activated mice were represented as CA-β-catenin. After 4 weeks, the tibia samples of mice were taken and the tibia metaphysis was scanned by CT scan and the data were analyzed, immunohistochemistry staining and ELISA were ben taken too. Results The tibia bone mass of the genetic mice were increased (P<0.05); the immunohistochemistry staining indicated that the TNAP positive number of osteoblasts in genetic mice were more than that in WT mice in each group (P<0.05). Elisa test results indicated that the bone formation indexes of CA-β-catenin mice were increased in each group, and the bone resorption-related indexes of CA-β-catenin mice were decreased in each group. The bone mass of senile genetic mice was still less than that of adult genetic mice (P<0.05), but the increase in bone mass of senile genetic mice was greater than adult mice (P<0.05); the number of osteoclasts in senile genetic mice was still more than that of adults group (P<0.05). Conclusion Continuous activation of β-catenin can increase bone mass in both adult and old mice, while the increase in bone mass of CA-β-catenin mice in the aged group is greater. |
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