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| 基于Wnt/β-catenin信号通路探讨太白楤木抗去卵巢大鼠骨质疏松症的作用和机制 |
| Effect and mechanism of aralia taibaiensis on anti-osteoporosis through Wnt/β-catenin signaling pathway in ovariectomized rats |
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| DOI：10.3969/j.issn.1006-7108.2022.05.005 |
| 中文关键词: 太白楤木 去卵巢大鼠 骨质疏松症 Wnt/β-catenin信号通路 |
| 英文关键词:aralia taibaiensis ovariectomized rats osteoporosis Wnt/β-catenin signaling pathway |
| 基金项目:国家自然科学基金项目(81703777)；陕西省自然科学基础研究计划（2021JM-471）；陕西中医药大学附属医院科研项目（2020QN006）；现代农业产业技术体系建设专项基金项目（CARS-21） |
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| 中文摘要: |
| 目的 探索太白楤木对去卵巢大鼠骨质疏松症以及Wnt/β-catenin信号通路的影响。方法 40 只雌性SD大鼠随机分为假手术组、模型组、仙灵骨葆胶囊组（0.3 g/kg）、太白楤木低剂量组（0.4 g/kg）和太白楤木高剂量组（0.8 g/kg），每组8 只，除假手术组外其余组进行去卵巢手术。术后灌胃给药12周，三点弯曲试验测定骨生物力学性能，Micro-CT检测骨密度和微结构，ELISA法检测血清骨代谢指标，HE染色观察胫骨病理改变，Western blot法测定骨组织中Wnt/β-catenin信号通路相关蛋白的表达情况。结果 经过12周治疗，与模型组相比，太白楤木高剂量组显著增大最大载荷、刚度和最大应力(P＜0.01)，提高去卵巢大鼠骨密度(P＜0.01)，保护骨小梁微结构不被破坏，骨吸收标志物NTX-Ⅰ和TRAP水平降低(P＜0.01)，骨形成标志物BALP水平升高(P＜0.01)，Wnt3a、β-catenin和RUNX2蛋白表达量升高(P＜0.01)，p-β-catenin蛋白水平降低(P＜0.01)。结论 太白楤木对去卵巢大鼠生物力学性能、骨密度和骨微结构具有保护作用，太白楤木调节骨代谢可能和激活Wnt/β-catenin信号通路有关。 |
| 英文摘要: |
| Objective To explore the effect and mechanism of aralia taibaiensis on osteoporosis through Wnt/β-catenin signaling pathway in ovariectomized rats. Methods Forty female SD rats were randomly divided into sham operation group, model group, Xianlinggubao capsule group (0.3 g/kg), aralia taibaiensis low-dose group (0.4 g/kg), and aralia taibaiensis high-dose group (0.8 g/kg). There were 8 rats in each group. Except for rats in the sham operation group, rats in other groups underwent ovariectomy. Corresponding drugs were intragastrically administered for 12 weeks after operation. Bone biomechanical properties were measured using three-point bending test. Bone mineral density and microstructure were detected with micro-CT. Serum bone metabolism indexes were detected with ELISA. Morphological changes of bone tissue was observed with HE staining. The expression of proteins in Wnt/β-catenin signaling pathway in bone tissue was detected by Western blotting. Results Compared to the model group, after 12 weeks of high-dose aralia taibaiensis treatment, the maximum load, stiffness, maximum stress and bone mineral density increased significantly (P<0.01). Aralia taibaiensis treatment protected bone trabecular microstructure from damage. The levels of bone resorption markers NTX-Ⅰ and TRAP decreased (P<0.01). However, the level of bone formation marker BALP increased (P<0.01). The protein expression of Wnt3a, β-catenin, and Runx2 increased significantly (P<0.01). p-β-catenin decreased significantly (P<0.01). Conclusion Aralia taibaiensis has protective effects on biomechanical properties, bone mineral density, and bone microstructure in ovariectomized rats. Aralia taibaiensis may regulate bone metabolism through activation of Wnt/β-catenin signaling pathway. |
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