| Objective To explore the mechanism of Jintiange capsule in prevention and treatment of osteoporosis by using metabolomic analysis. Methods The rats were ovariectomized to establish osteoporosis model, and divided into four groups, including sham control group, OVX model group, Jintiange low dose group (JTG - 180 mg/kg) and Jintiange high dose group (JTG - 360 mg/kg). The rats were orally administrated for 12 weeks, and the bone microstructure were analyzed by Micro-CT analysis, and the serum metabolites were detected by LC-MS spectrometry, the potential biomarkers related to osteoporosis were screened and identified by multivariate statistical analysis and univariate statistical analysis. Results Micro-CT analysis showed that Jintiange capsule could significantly improve the bone microstructure, and increase the bone mineral density (BMD) and bone mineral content (BMC) in ovariectomized rats. Metabolomics analysis exhibited that there had significant altered trajectory in serum metabolites of ovariectomized rats, and seventeen potential biomarkers related to osteoporosis were identified in serum, involving tryptophan metabolism, vitamin B6 metabolism, retinol metabolism, glycerol phospholipid metabolism, primary bile acid biosynthesis, amino acyl-tRNA biosynthesis. Jintiange was involved into tryptophan metabolism, vitamin B6 metabolism, glycerol phospholipid metabolism and aminoacyl-tRNA biosynthesis. Conclusion Jintiange capsule exerts anti-osteoporotic effects through involving into the regulation of vitamin B6 and tryptophan metabolism. |