| Objective To explore the role of dihydroflavonoids of psoraleae fructus in the differentiation of osteoclasts. Methods RANKL+M-CSF was used to induce bone marrow monocytes to differentiate into osteoclasts. During the differentiation process, isobavachin, bavachin, bavachinin were additioned for intervention. After 3 days, the EC50 was calculated according to the anti-tartrate acid phosphatase activity of each group of cells. The compound with the strongest inhibitory effect was selected for the next test. Western blotting was used to analyze the levels of key proteins in the NF-κB signaling pathway, PI3k/AKT signaling pathway, and MAPK signaling pathway in osteoclasts under the intervention of bavachinin. Results The EC50 of isobavachin, bavachin, and bavachinin was 15.89, 15.18, and 12.39 μmol/L, respectively. Bavachinin significantly down-regulated the phosphorylation levels of p65, AKT, p38, JNK, and ERK during osteoclast differentiation. Conclusion Bavachinin has the strongest inhibitory effect on osteoclast differentiation. Its mechanism of action is related to the inhibition of the activation of NF-κB signaling pathway, PI3k/AKT signaling pathway, and MAPK signaling pathway. This study provides reference for the clinical application of bavachinin in the treatment of osteoporosis. |