中国骨质疏松杂志

当归鸡血藤化裁方发挥类雌激素作用保护去卵巢大鼠骨量流失

Flavored angelica caulis spatholobi decoction exerts estrogen-like effects to protect bone loss in ovariectomized rats

DOI：10.3969/j.issn.1006-7108.2022.07.017

中文关键词: 骨质疏松症当归鸡血藤化裁方雌激素高骨转换 OPG/RANKL/RANK通路 ERK通路

英文关键词:osteoporosis flavored angelica caulis spatholobi decoction estrogen high bone turnover rate OPG/RANKL/RANK pathway ERK pathway

基金项目:河北省院士工作站建设专项资助(199A7742H)

作者	单位
余旭东1 刘淑刚2 高晓璐2 陈虎1，3*	1.河北中医学院，河北石家庄 050090 2.河北医科大学第四医院，河北石家庄 050011 3.河北省中医药科学院，河北石家庄 050030

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中文摘要:

目的观察当归鸡血藤化裁方对去卵巢大鼠骨密度和骨量的影响并探讨相关的作用机制。方法将30只3月龄雌性大鼠随机分为Sham组、OVX组以及OVX+T组，每组10只；适应性饲养两周后对Sham组行假手术，对OVX及OVX+T组行双侧卵巢去势手术，其中OVX+T组于术后两个月给予等效当归鸡血藤化裁方灌胃治疗；连续给药12周后，取各组大鼠血清及股骨组织分别进行骨密度检测、组织切片检测、蛋白印迹检测及血清指标检测。结果 X线及HE切片显示，OVX组骨密度及骨微参数均低于Sham组，OVX+T组骨密度及骨微参数高于OVX组(P＜0.05)。血清指标显示，OVX组的17β-E2水平低于Sham组，ALP、CTX-1、TRAcP-5b含量或活性高于Sham组；OVX+T组的17β-E2水平高于OVX组，ALP、CTX-1、TRAcP-5b含量或活性低于OVX组(P＜0.05)。蛋白印迹结果显示，OVX组的OPG、ERK表达水平低于Sham组，RANKL、RANK、RANKL/OPG的比率高于Sham组；OVX+T组的OPG、ERK表达水平高于OVX组，RANKL、RANK、RANKL/OPG的比率低于OVX组(P＜0.05)。结论当归鸡血藤化裁方是通过类雌激素作用抑制骨的高转化率，并通过OPG/RANKL/RANK信号通路来促进骨形成并抑制骨吸收，从而来防治雌激素缺乏引起的骨质疏松症。在这一过程中可能有ERK信号通路的参与。

英文摘要:

Objective To observe the effect of flavored angelica caulis spatholobi decoction on bone mineral density and bone mass in ovariectomized rat models, and to explore the related mechanism and signal pathways. Methods Thirty 3-month-old female rats were randomly divided into Sham group, OVX group, and OVX+T group, with 10 rats in each group. After two weeks of adaptive feeding, ovariectomy for both ovaries was performed for rats in OVX and OVX+T group, while rats were sham operated in Sham group. Rats in OVX+T group received equivalent flavored angelica caulis spatholobi decoction through gavage two months after the operation. After continuous administration for 12 weeks, the serum and femoral tissues of rats in each group were collected for bone mineral density (BMD) test, tissue section test, Western blotting test, and serum index test. Results X-rays and HE sections showed that BMD and bone microparameters in OVX group were lower than those in Sham group, and BMD and bone microparameters in OVX+T group were higher than those in OVX group (P<0.05). Serum indicators show that the level of 17β-E2 in OVX group was lower than that in Sham group, and the content or activity of ALP, CTX-1, and TRAcP-5b were higher than those in Sham group. The level of 17β-E2 in OVX+T group was higher than that in OVX group, and the content or activity of ALP, CTX-1 and TRAcP-5b were lower than those in OVX group (P<0.05). Western blotting results showed that the expression levels of OPG and ERK in OVX group were lower than those in Sham group, and the ratios of RANKL, RANK, and RANKL/OPG were higher than those in Sham group. The expression levels of OPG and ERK in OVX+T group were higher than those in OVX group, and the ratio of RANKL, RANK, and RANKL/OPG was lower than that in OVX group (P<0.05). Conclusion The study shows that flavored angelica caulis spatholobi decoction is most likely to inhibit high bone conversion rate through estrogen-like effect, and to promote bone formation and to inhibit bone absorption through OPG/RANKL/RANK signaling pathway, so as to prevent and treat osteoporosis caused by estrogen deficiency. ERK signaling pathway may be involved in this process.

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