| Abstract: Objective From the phosphatidylinositol 3-kinase/serine-threonine kinase (PI3K/AKT) pathway, to observe the effects of intestinal flora regulator-probiotic preparations on bone metabolism and bone mass, and to provide reliable information for probiotics to treat osteoporosis (OP). Methods The intestinal flora dysbiosis OP model was established and set as: normal control group, model group, probiotic preparation group, PI3K/AKT pathway activator group, probiotic preparation + pathway activator group, with 15 animals in each group. After 14 days of drug intervention, the water content and flora distribution of feces were detected; the changes of serum inflammatory factors and bone biological indexes were detected by ELISA; the bone mineral density was detected by absorptiometry; (TRACP) staining was used to observe the number of bone pathological damage and osteoclast bone resorption; Western blot was used to detect the expression of PI3K/Akt pathway-related proteins. Results Compared with the normal control group, the rats in the model group showed an imbalance of intestinal flora, increased serum inflammatory factors, abnormal bone metabolism (decreased osteogenesis factors, decreased osteoclastic activities such as β-CTX and RANKL), and PI3K/Akt pathway. The activity increased (P<0.05). Probiotic preparations can correct the imbalance of intestinal flora, inhibit the release of serum inflammatory factors, improve the imbalance of bone metabolism in OP rats, and inhibit the activity of PI3K/Akt pathway (P<0.05). PI3K/Akt pathway activators can weaken the above effects of probiotic preparations, aggravate the imbalance of bone metabolism and intestinal flora in OP (P<0.05) Conclusion Intervention treatment with probiotic preparations can attenuate the dysbacteriosis of rats and correct the abnormal OP bone metabolism, and its effect may be related to the inhibition of PI3K/Akt pathway activation. |