| Abstract: Objective To study the effect of total flavonoids of rhizoma drynariae on osteogenic differentiation of mesenchymal stem cells (MSCs) derived from induced pluripotent stem cells (iPSCs). Methods The total flavonoids of rhizoma drynariae were diluted to concentrations of 15.63, 31.25, 62.5, 125, and 250 μg/mL, respectively, and added to the cell culture medium. The medium did not contain total flavonoids was as the control group. iPS-MSCs were inoculated into different concentrations of culture medium. CCK8 method was used to detect the proliferation of iPS-MSCs in each group. ALP activity detection and Alizarin red staining method were used to detect the bone differentiation of each components. RT-PCR was used to detect the mRNA expressions of OCN and collagen I in each group. Results CCK8 results showed that the total flavonoids of rhizoma drynariae at the concentration of 125 μg/mL and 250 μg/mL inhibited the growth of iPS-MSCs. Therefore, the concentration of 62.5 μg/mL or less was selected to study the differentiation of iPS-MSCs. After cultured in osteogenic induction medium, the activity of ALP in the three treatment groups was higher than that in the 0 μg/mL blank control group, the calcified nodules were increased with Alizarin red staining, and the difference was statistically significant (P<0.0l). In addition, RT-PCR results showed that the mRNA expressions of OCN and collagen I in the three concentration groups were significantly higher than those in the control group. Conclusion Total flavonoids of rhizoma drynariae promotes the osteogenic differentiation of iPS-MSCs. Its mechanism may be related to the increased expressions of OCN and collagen I, the key factors for osteogenesis. |