| Abstract: Objective To explore the therapeutic mechanism of Jingang Pill with the function of reinforcing kidney essence to strengthen bone on ovariectomized(OVX) rat osteoporosis based on mRNA expression of Hippo signal pathway core genes. Methods Postmenopausal Osteoporosis(PMOP) rat model was established with ovariectomy. All the rats were divided into 8 groups: normal group, sham-operated group, model group, Jingang Pill high-dose group,Jingang Pill middle-dose group, Jingang Pill low-dose group, Xianlinggubao control group and calcitriol control group.After 12 weeks of drug administration,bone mineral density(BMD) was detected using X-ray bone density apparatus,microstructure of femoral head was observed with microscope, serum ALP was detected using ELISA and mRNA expression of Mst2,Lats1,Taz in bone was detected using real-time quantitative RT-PCR. Results ①Compared to those in normal group,bone microstructure in model group damaged significantly, femur BMD, serum ALP, mRNA expression of Taz in bone in model group decreased significantly (P＜0.01), and mRNA expression of Mst2, Lats1 in bone in model group increased significantly(P＜0.01).②Compared to those in model group, the damage of bone microstructure improved in all treatment groups, BMD increased significantly(P＜0.01) in all treatment groups except Jingang Pill low-dose group,serum ALP,mRNA expression of Taz in bone increased significantly(P＜0.01) in all treatment groups,and mRNA expression of Mst2, Lats1 in bone decreased significantly(P＜0.01) in all treatment groups.The most significant difference was found in Jingang Pill high-dose group. Conclusion The mRNA expression of mst2 and LATS1, the core genes of Hippo signaling pathway, is up-regulated in bone tissue, and the down-regulation of TAZ mRNA may be one of the pathogenesis of PMOP; Jingang pill may effectively prevent and treat PMOP by down regulating the expression of core genes mst2 and LATS1 mRNA of Hippo signal pathway in bone tissue and up regulating the expression of TAZ mRNA. |