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| 脂多糖对小鼠胫骨骨髓去除后早期骨形成的影响 |
| Effect of lipopolysaccharide on early bone formation after tibial bone marrow ablation in mice |
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| DOI：10.3969/j.issn.1006.7108.2022.09.010 |
| 中文关键词: 脂多糖 骨髓去除模型 骨形成 成骨分化 Wnt/β-catenin信号 |
| 英文关键词:lipopolysaccharide bone marrow ablation bone formation osteogenesis Wnt/β-catenin signal |
| 基金项目:国家自然科学基金 (81802206)；军队保健专项课题面上项目（19BJZ11）；西部战区总医院院管课题（2021-XZYG-C11） |
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| 中文摘要: |
| 目的 利用小鼠胫骨骨髓去除模型（bone marrow ablation, BMX），观察脂多糖（lipopolysaccharide，LPS）对BMX后早期骨形成的影响，并初步探讨其机制。方法 建立小鼠胫骨BMX模型后，分为对照组（PBS处理）和LPS处理组，每组6只。BMX后1周，通过组织病理切片染色和组织形态计量观察胫骨骨髓腔中骨形成情况。通过免疫组化检测BMX后4 d骨髓腔中增殖细胞核抗原（PCNA）表达情况，BMX后1周β-catenin和Runx2表达情况。采用RT-PCR检测BMX后1周新生骨组织中成骨分化相关基因和Wnt/β-catenin信号通路下游靶基因表达水平。结果 BMX后1周，LPS处理组胫骨骨髓腔中新生骨组织较对照组减少，LPS处理组新生骨组织BV/TV较对照组显著减少；BMX后4 d，LPS处理组PCNA阳性细胞较对照组明显减少；BMX后1周，LPS处理组新生骨组织中Runx2阳性细胞和β-catenin阳性细胞较对照组明显减少；BMX后1周，LPS处理组新生骨组织中成骨分化相关基因和Wnt/β-catenin信号通路下游靶基因表达水平较对照组明显降低。结论 LPS可以抑制间充质细胞增殖，抑制成骨分化，导致BMX后早期骨形成减少，Wnt/β-catenin信号通路可能参与了LPS抑制BMX后早期骨形成的过程。 |
| 英文摘要: |
| Objective To investigate the effect of lipopolysaccharide (LPS) on early bone formation after bone marrow ablation (BMX) in mouse tibia, and to investigate its mechanism. Methods Mouse tibia BMX model was established and divided into control group (PBS treatment) and LPS treatment group. One week after BMX, new bone formation in tibial bone marrow cavity was observed by histopathological staining and histomorphometry. The expression of proliferating cell nuclear antigen (PCNA) in bone marrow cavity 4 days after BMX and the expression of β-catenin and Runx2 one week after BMX were detected with immunohistochemistry. RT-PCR was used to detect the mRNA levels of osteogenic differentiation related genes and downstream target genes of Wnt/β-catenin signaling pathway in new formed bone tissue 1 week after BMX. Results One week after BMX, the new bone tissue in tibial bone marrow cavity after LPS treatment was reduced. BV/TV of new bone tissue after LPS treatment was significantly reduced compared with the control group. Four days after BMX, PCNA positive cells in bone marrow cavity were significantly reduced after LPS treatment compared with control group. One week after BMX, Runx2-positive osteoblasts on the surface of newborn bone tissue of LPS-treated mice were significantly reduced compared with control mice. One week after BMX, the expression levels of osteogenic differentiation related genes and downstream target genes of Wnt/β-catenin signaling pathway in the LPS-treated group were significantly reduced compare with control group. Conclusion LPS inhibits mesenchymal cell proliferation and osteogenic differentiation, leading to reduced bone formation after BMX, possibly through negative regulation of Wnt/β-catenin signaling pathway. |
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