| Objective To observe the effect of Jiawei Yanghe Decoction-containing serum on the co-cultivation system of MC3T3-E1 and RAW264.7 cells, and to explore its possible mechanism of the prevention and treatment of osteoporosis (OP) with kidney-yang deficiency syndrome. Methods Prepare medicated serum of Jiawei Yanghe Decoction, the MC3T3-E1 and RAW264.7 cell co-cultivation system was established on the Transwell co-culture plate, and the kidney-yang deficiency serum group, blank group, drug-containing serum + kidney-yang deficiency serum group and drug-containing serum group were set up. After the intervention of the co-cultivation system for 48 hours, Alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) staining were used to observe osteogenic and osteoclastic differentiation. TRAP and ALP kits were used to detect TRAP in the cell supernatant. And ALP activity, Western blot detected the expression of OPG, RANKL, RANK protein in each group. Results Compared with the kidney-yang deficiency serum group, the ALP activity of the drug-containing serum + the kidney-yang deficiency serum group increased (P<0.05), the TRAP activity and the number of OC decreased (P<0.05, P<0.05), and the expression of OPG protein was up-regulated (P<0.05, P<0.05), the expression of RANKL and RANK protein was down-regulated (P<0.05, P<0.05). Conclusion Jiawei Yanghe Decoction has corresponding therapeutic effect on OP kidney-yang deficiency syndrome, and its mechanism may be related to the increase of OPG and the decrease of RANKL and RANK protein expressions. |