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| 3D细胞培养和类器官在骨髓源性间充质干细胞成骨分化中的研究进展 |
| Research progress in 3D cell culture and organoids of osteogenic differentiation in bone marrow mesenchymal stem cells |
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| DOI：10.3969/j.issn.1006.7108.2022.09.028 |
| 中文关键词: 类器官 间充质干细胞 成骨分化 组织工程 3D细胞培养 |
| 英文关键词:organoid bone marrow mesenchymal stem cells osteogenesis differentiation tissue engineering 3D culture |
| 基金项目:国家自然科学基金（81972156）；辽宁省自然科学基金（2019-ZD-0781） |
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| 中文摘要: |
| 类器官（organoids）来源于自体的组织及干细胞，是通过体外3D培养后形成的细胞团块，这种团块具有原始组织及器官的三维结构，并保留了相对应的功能和遗传特征。由于其具有模拟特定机体器官的发育和疾病发生发展的潜能，这一模型在多种药物的筛选和分子机制研究中拥有更多的优势。近年来，已有实验表明骨髓源性间充质干细胞（BMSCs）通过3D培养及成骨分化可以形成骨的类器官，并可以植入机体发挥特定的作用。这种骨类器官模型的构建，不仅可以为骨质疏松等相关疾病研究提供更多方法，还在骨组织移植及修复等组织工程学中发挥重要作用。本文就BMSCs成骨分化的类器官相关3D模型研究进展作一综述，为BMSCs成骨分化的类器官的基础和临床研究提供更多理论依据和思路。 |
| 英文摘要: |
| Organoids are derived from autologous tissues and stem cells and formed by 3D culture in vitro. These cells have the three-dimensional (3D) structure of original tissues and organs while retaining the corresponding functions and genetic characteristics. Because of its potential to simulate the development of specific body organs and the occurrence and development of diseases, this model has more advantages in the screening of a variety of drugs and the study of molecular mechanisms. In recent years, many studies have shown that bone marrow-derived mesenchymal stem cells (BMSCs) can form bone-like organs through 3D culture and osteogenic differentiation, and can be implanted into the body to play specific roles. The construction of such bone organ model not only provides more methods for the study of osteoporosis and other related diseases, but also plays an important role in tissue engineering, such as bone tissue transplantation and repair. In this review, we overview the progress of organoid 3D models of osteogenic differentiation in BMSCs, providing more theoretical basis and ideas for basic and clinical studies on organoids of osteogenic differentiation in BMSCs. |
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