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| 骨质疏松性骨折与非骨质疏松性骨折患者肌肉病理状态及组蛋白甲基化的差异 |
| Differences in muscle pathological status and histone methylation between patients with osteoporotic and non-osteoporotic fractures |
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| DOI：10.3969/j.issn.1006.7108.2022.10.001 |
| 中文关键词: 骨质疏松骨折 非骨质疏松骨折 肌肉 病理 组蛋白甲基化 |
| 英文关键词:Osteoporotic fractures non-osteoporotic fractures muscle pathology histone methylation |
| 基金项目:国家重点研发计划项目（2018YFC1704300）；国家自然科学基金项目（81973883，81730107）；上海市自然科学基金项目（19ZR1458000）；上海市科委中药创新与传承领域项目（20S21902000） |
| 作者 | 单位 | | 王乾1,2 孙攀1,2 黄廷锐1,2 黄晨1,2 万宏波1 赵永见1,2,3 邬学群1 王拥军1,2,3 施杞1,2,3
唐德志1,2,3* | 1.上海中医药大学附属龙华医院， 上海 200032
2.上海中医药大学脊柱病研究所，上海 200032
3.教育部筋骨理论与治法重点实验室，上海 200032 |
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| 中文摘要: |
| 目的 评估骨质疏松性骨折（osteoporotic fracture，OPF）患者与非OPF患者肌肉病理状态及组蛋白甲基化情况的差异。方法 从上海中医药大学附属龙华医院骨伤科随机收取6例符合纳入标准的OPF和非OPF患者，手术内固定时取骨折部位肌肉，进行HE染色、MASSON染色、免疫组化染色及RT-qPCR检测，评估OPF患者和非OPF患者的肌肉病理状态差异和组蛋白甲基化情况。结果 HE染色显示OPF患者肌肉与非OPF患者肌肉相比，肌肉松弛现象比较明显，而非OPF患者肌肉较紧实。MASSON染色显示OPF患者肌肉与非OPF患者肌肉相比，肌肉胶原纤维分布更多。骨折断端肌肉RT-qPCR显示OPF患者较非OPF患者SUV39H1基因相对表达量增高。骨折断端肌肉免疫组化显示OPF患者较非OPF患者肌肉H3K9Ac乙酰化程度低，肌肉H3K9me3甲基化程度高。结论 骨质疏松性骨折患者肌肉更加松弛，胶原纤维含量增高，而这可能是由于肌肉组蛋白甲基化程度升高，乙酰化降低造成的。 |
| 英文摘要: |
| Objective Assessment of muscle pathology and histone methylation in patients with osteoporotic fracture (OPF). Methods Six patients with OPF and non-OPF who met the inclusion criteria were randomly collected from the Department of Orthopedic Injuries, Longhua Hospital, Shanghai University of Traditional Chinese Medicine. The muscles at the hip fracture site were taken at the time of surgical internal fixation, and HE staining, MASSON staining, immunohistochemical staining and RT-qPCR were performed to assess the differences in muscle pathological status and histone methylation between patients with OPF and non-OPF. Results HE staining showed more muscle laxity in OPF patients' muscles compared with non-OPF patients' muscles, while non-OPF patients' muscles were tighter. MASSON staining showed more muscle collagen fiber distribution in OPF patients' muscles compared with non-OPF patients' muscles. RT-qPCR of fracture end muscles showed an increased relative expression of SUV39H1 gene in OPF patients compared to non-OPF patients. Immunohistochemistry of fracture severed end muscles showed lower muscle H3K9Ac acetylation and higher muscle H3K9me3 methylation in OPF patients compared to non-OPF patients. Conclusion Patients with osteoporotic fractures have more flaccid muscles and higher collagen fiber content, which may be due to elevated muscle histone methylation and reduced acetylation. |
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