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| 金天格胶囊对H2O2诱导的小鼠成骨细胞MC3T3-E1氧化应激损伤及炎症因子的作用 |
| Effect of Jintiange Capsule on osteoblast MC3T3-E1 induced by H2O2 through inhibiting oxidative stress damage and the release of inflammatory cytokines in vitro |
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| DOI：10.3969/j.issn.1006.7108.2022.10.008 |
| 中文关键词: 骨质疏松 金天格胶囊 成骨细胞 碱性磷脂酶 Ⅰ型前胶原羧基端前肽 |
| 英文关键词:osteoporosis Jintiange capsule osteoblast ALP PICP |
| 基金项目:云南省教育厅科学研究基金项目（2020J0574） |
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| 中文摘要: |
| 目的 研究金天格胶囊对骨质疏松中氧化应激损伤和炎症因子释放的改善作用。方法体外培养MC3T3-E1小鼠成骨细胞，分别加入10、20、50和100 μg/mL金天格胶囊溶液，测定药物作用不同时间后MC3T3细胞存活率，和细胞培养上清液中ALP和PICP含量。利用1 mmol/L H2O2建立MC3T3细胞诱导损伤模型，给予不同浓度金天格胶囊，测定细胞培养上清液中SOD、GSH、CAT和MDA含量和TNF-α、IL-6及IL-1β水平，然后测定MC3T3细胞中Tnfa、Il-6、Il-1b mRNA相对表达。结果 与空白组比较，100 μg/mL金天格胶囊能显著提高MC3T3-E1细胞存活率，50、100 μg/mL金天格胶囊能显著增加细胞培养上清中ALP和PICP含量。在H2O2刺激MC3T3-E1细胞损伤模型中，50、100 μg/mL金天格胶囊能显著提高H2O2诱导损伤后细胞存活率，并能明显增加细胞培养上清液中SOD、GSH、CAT活力，降低MDA水平；此外，与H2O2组相比，50、100 μg/mL金天格胶囊能够显著降低MC3T3细胞培养上清中TNF-α、IL-6含量，显著降低细胞中Tnfa、Il-6 mRNA相对表达，100 μg/mL金天格胶囊能降低Il-1b mRNA相对表达。结论 金天格胶囊能促进小鼠成骨细胞MC3T3-E1增殖，并抑制由H2O2引起的炎症基因表达和炎症因子释放。 |
| 英文摘要: |
| Objective To study the amiliorative effect of Jintiange Capsule on osteoporosis by inhibiting oxidative stress injury and inflammatory cytokines release. Methods Mouse osteoblast MC3T3-E1 was cultured and incubated by 10, 20, 50 and 100 μg/mL Jintiange Capsule solution. Then MC3T3-E1 cell viability and contents of ALP and PICP in cultural supernatant were determined. 1 mmol/L H2O2 was used to induce MC3T3-E1 cell injury model. The injury MC3T3-E1 cell was treated by different concentrations of Jintiange Capsule. Then the contents of oxidative factor MDA and anti-oxidative factors SOD, GSH-Px and CAT and cytokines TNF-α, IL-6 and IL-1β in cultural supernatant were assayed. The relative mRNA expression of Tnfa, Il6 and Il1b in MC3T3-E1 was determined. Results Compared with control group, 100 μg/mL Jintiange Capsule could improve cell viability of MC3T3-E1, both 50 and 100 μg/mL Jintiange Capsule could increase contents of ALP and PICP in cultural supertanant. In the MC3T3-E1 cell injury model induced by H2O2 50 and 100 μg/mL Jintiange Capsule could increase cell viability and improve the activities of SOD, GSH-Px and CAT, decrease level of MDA. Compared with H2O2 group, 50 and 100 μg/mL Jintiange Capsule could reduce contents of TNF-α and IL-6 in cultural supertanant and lower relative mRNA expression of Tnfa and Il6, 100 μg/mL Jintiange Capsule could decrase relative mRNA expression of Il1b. Conclusion Jintiange Capsule could improve cell proliferation and inhibit inflammatory gene expression and inflammatory cytokines release in MC3T3-E1 induced by H2O2. |
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