| Objective To investigate the effect of flavonoids extract of Cockscomb on tMAO-NF -κB/NFATc1 level, bone microstructure and bone specific phosphatase protein in aged osteoporosis rats. Methods 16-week-old rats were divided into sham operation group (SHAM group, n=13), model group (n=9), intervention group (n=11) and drug control group (n=11). OP model was established in all rats except sham group. Results Compared with sham group, serum LEVELS of Ca, P, ALP and BAP in model group were decreased, and there were differences (P < 0.05); compared with model group, serum levels of Ca, P, ALP and BAP in intervention group were increased, and there were differences (P < 0.05); BMD of sham group, model group, intervention group and drug control group were (58.05±6.11) mg/cm3, (47.50±4.53) mg/cm3, (55.17±5.14) mg/cm3 and (54.96±5.40) mg/cm3, and there were differences among groups (F=6.983, P < 0.001). Compared with sham group, TMAO, NF-κB and NFATc1 expressions were higher in model group than in sham group (P < 0.05), while TMAO, NF-κB and NFATc1 expressions were lower in intervention group than in model group (P < 0.05). Conclusion The intervention of flavonoids extract of C. cristatum can significantly improve bone microstructure, increase bone-specific phosphatase activity and increase bone mineral density in aged rats with osteoporosis. The mechanism may be related to the inhibition of TMAO, NF-κB and NFATc1 expression. |