| Objective To explore the effect of quercetin on autophagy regulation mechanism of osteoclast differentiation. Methods The experiment was divided into control group , RANKL group and RANKL + quercetin group. CCK8 was used to detect the concentration of quercetin. After 7 days of induction, the model was verified by TRAP staining. After 48 hours of quercetin intervention, TRAP staining was performed, and qPCR was used to detect the gene expression of TRAP, CTSK, NFATC-1, ATG5, p62, mTOR and Beclin-1. Results Quercetin 1mg / L was selected according to CCK8 results. TRAP staining showed that osteoclasts increased significantly in RANKL group, and osteoclasts decreased after quercetin intervention. qPCR results showed that the gene expression of TRAP, P62, CTSK and NFATC-1 increased, while the gene expression of ATG5, Beclin-1 and mTOR decreased in the model group. The gene expression of TRAP, CTSK, NFATC-1, ATG5, p62, mTOR and Beclin-1 decreased in the quercetin intervention group. Electron microscopic observation showed that the number of autophagy bodies in osteoclasts decreased after quercetin intervention. Conclusion Quercetin can inhibit osteoclast differentiation by reducing the expression level of autophagy genes. |