| Objective The purpose of this study is to observe the effect of naringin on HIF-1α/VEGF signaling pathway and H-type angiogenesis in ovariectomized rats, and to explore the mechanism of anti-osteoporosis of naringin. Methods Thirty-six rats were randomly divided into three groups: ovariectomized group, sham group, and naringin treatment group. Rat model of osteoporosis was established with bilateral ovariectomy. After 3 months of naringin treatment, the changes of bone metabolism in serum and bone marrow supernatant, HIF-1α/VEGF signaling pathway, bone mineral density (BMD), bone microstructure and H-type vessels were detected. Results Compared to the ovariectomized group, the indicator of osteogenic metabolism including BALP, BGP, PINP were increased and CTX was decreased in serum in naringin treatment group. HIF-1 α expression was increased in both serum and bone marrow. VEGF expression was increased in bone marrow, but not in serum. Micro-CT results showed that BMD and bone mass were increased in naringin treatment group. Corresponding, increased H-vessels in the proximal tibia were observed in naringin treatment group compared with that of ovariectomized group. Conclusion Naringin can regulate bone metabolism, improve BMD and play an anti-osteoporosis role in ovariectomized rats, and this may be achieved by promoting H-type angiogenesis through HIF-1 α/VEGF signaling pathway. |