| Objective To observe the concentration of IL-1β in synovial fluid and the expression of MMP-13 in articular cartilage tissue of KOA rats after intervention with different doses of Vitamin D2, and to explore their effects on inflammatory response and cartilage degradation. Methods Fifty female SD rats aged 2-3 months were selected, and the KOA rat model was prepared by intra-articular injection of papain. The rats were randomly divided into group A (model group) and group B (4 × 105 U/kg), group C (5 × 105 U/kg), group D (6 × 105 U/kg) and group E (7 × 105 U/kg), 10 in each group. Except for group A, the other four groups were given Vitamin D2 intervention once a week for 4 consecutive weeks. The rat knee joint tissue was taken to prepare sections, HE staining, toluidine blue staining, modified safranin O-fast green staining and Mankin’s score were performed to detect the concentration of IL-1β in synovial fluid and the expression of MMP-13 in cartilage tissue of rats Happening. Results After the intervention of different doses of Vitamin D2, chondrocytes increased, matrix staining was deepened, cartilage layer thickened, Mankin’s score, IL-1β concentration in synovial fluid and MMP 13 expression level in cartilage tissue were all lower than those in the model group ( P<0.05). Among them, the cartilage layer thickening in the Vitamin D2 dose group of 6 × 105 U/kg was significantly thicker than that in other Vitamin D2 dose groups, and the Mankin’s score, the concentration of IL-1β in synovial fluid and the expression level of MMP-13 in cartilage tissue were lower than those in the Vitamin D2 dose group. Other Vitamin D2 dose groups. Conclusion Vitamin D2 can reduce the inflammatory response in joints, reduce cartilage matrix degradation, and promote articular cartilage repair by inhibiting the expression levels of IL-1β and MMP-13. And the intervention effect of Vitamin D2 at a dose of 6 × 105 U/kg was better. |