中国骨质疏松杂志

朝藿定C联合骨髓间充质干细胞移植对糖皮质激素性骨质疏松小鼠骨密度和骨代谢的影响

Effects of chaohuoding C combined with bone marrow mesenchymal stem cell transplantation on bone mineral density and bone metabolism in glucocorticoid-induced osteoporosis mice

DOI：10.3969/j.issn.1006.7108.2023.04.003

中文关键词: 骨质疏松糖皮质激素朝藿定C 骨髓间充质干细胞骨密度骨代谢 PI3K/AKT通路

英文关键词:osteoporosis glucocorticoid chaohuoding c bone marrow mesenchymal stem cells bone mineral density bone metabolism PI3K/AKT pathway

基金项目:

作者	单位
陈登环* 帅宗文	安徽医科大学第一附属医院（安徽省儿童医院）风湿免疫科，安徽合肥 230000

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中文摘要:

目的探讨朝藿定C联合骨髓间充质干细胞移植对糖皮质激素性骨质疏松小鼠骨密度和骨代谢的影响。方法 75只雄性C57BL/6小鼠分为空白对照组、模型组、朝藿定C组、干细胞移植组、联合组，每组各15只。空白对照组以0.1 mL生理盐水肌肉注射，其余4组以0.1mL地塞米松肌肉注射，2次/周，连续干预8周，以骨密度值确定造模成功。造模成功后7 d分别进行骨髓间充质干细胞移植和朝藿定C灌胃治疗，连续8周。测定骨密度和骨结构参数、血清骨代谢指标水平以及AKT蛋白磷酸化（p-AKT）水平。结果与空白对照组比较，模型组体质量、骨矿物质含量（BMC）、骨矿物质密度（BMD）、组织矿物质含量（TMC）、组织矿物质密度（TMD）、骨体积分数（BV/TV）、骨小梁数量（Tb.N）、骨小梁厚度（Tb.Th）以及骨源性碱性磷酸酶（BALP）、Ⅰ型前胶原羧基端前肽（PINP）、AKT蛋白磷酸化水平显著降低，骨小梁分离度（Tb.Sp）显著增加，血清骨钙素（OCN）和抗酒石酸酸性磷酸酶（Trap）水平显著升高（P＜0.05）。与模型组比较，朝藿定C组、干细胞移植组和联合组小鼠体质量、BMC、BMD、TMC、TMD、BV/TV、Tb.N、Tb.Th显著增加，BALP、PINP、AKT蛋白磷酸化水平显著升高，Tb.Sp、血清OCN和Trap水平显著降低，且联合组上述指标改善优于其余2组（P＜0.05）。结论朝藿定C联合骨髓间充质干细胞移植可促进糖皮质激素性骨质疏松小鼠骨形成，增加骨密度，改善骨微结构，其机制可能与提高骨代谢水平和活化PI3K/AKT通路有关。

英文摘要:

Objective To investigate the effects of epimedin C combined with bone marrow mesenchymal stem cell transplantation on bone mineral density and bone metabolism in glucocorticoid-induced osteoporosis mice. Methods 75 male C57BL/6 mice were divided into blank control group, model group, epimedin C group, stem cell transplantation group and combination group, with 15 mice in each group. The control group was given intramuscular injection of 0.1mL normal saline, and the other four groups were given intramuscular injection of 0.1mL dexamethasone twice a week for 8 weeks. Bone mineral density, bone structure parameters, serum bone metabolism indexes and AKT protein phosphorylation (p-AKT) were measured. Results Compared with the blank control group, Body weight, bone mineral content (BMC), bone mineral density (BMD), tissue mineral content (TMC), tissue mineral density (TMD), bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), bone alkaline phosphatase (BALP), N-terminal propeptide of type I procollagen (PINP) and p-AKT of the model group mice decreased significantly, the trabecular separation/spacing (Tb.Sp) in the model group increased significantly, the levels of serum osteocalcin (OCN) and tartaric acid-resistant phosphatase (Trap) in model group were significantly higher (P<0.05). Compared with the model group, the body weight, BMC, BMD, TMC, TMD, BV/TV, Tb.N, Tb.Th of the mice in epimedin C group, stem cell transplantation group and combined group were significantly increased, the phosphorylation levels of BALP, PINP and AKT protein were significantly increased, and the levels of Tb.Sp, serum OCN and Trap were significantly decreased, and the improvement of the above indexes in combined group was better than that in the other two groups (P<0.05). Conclusion Hopidine C combined with bone marrow mesenchymal stem cell transplantation can promote bone formation, increase bone density and improve bone microstructure in glucocorticoid-induced osteoporosis mice, and its mechanism may be related to increasing bone metabolism level and activating PI3K/AKT pathway.

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