中国骨质疏松杂志

补肾活血汤防治骨质疏松症的作用机制

The mechanism of reinforcing kidney and activating blood decoction in the prevention and treatment of osteoporosis

DOI：10.3969/j.issn.1006-7108.2023.05.008

中文关键词: 补肾活血汤骨质疏松 TLR4/MyD88/NF-κB信号轴

英文关键词:reinforcing kidney and activating blood decoction osteoporosis TLR4/Myd88/NF-ΚB signal axis

基金项目:国家自然科学基金项目（81774339）；广东省中医药管理局面上项目（20191099）

作者	单位
麦文秀1* 谢雨欣2 张钰玲2 朱肇森2 唐宏宇1	1.广州中医药大学第一附属医院，广东广州 510405 2.广州中医药大学，广东广州 510405

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中文摘要:

目的基于TLR4/MyD88/NF-κB信号轴揭示补肾活血汤防治骨质疏松症的作用。方法 40只健康SD大鼠，随机分为假手术组、模型组、补肾活血汤组以及阿仑膦酸钠组，每组10 只，除假手术组外，其余各组造模后，补肾活血汤组予以补肾活血汤10 mL/(kg·d)灌胃，阿仑膦酸钠组给予阿仑膦酸钠片7.35 mg/(kg·d)灌胃，假手术组与模型组给予等量生理盐水灌胃，干预12周后取得大鼠骨组织，分析大鼠骨密度（bone mineral density，BMD）、骨矿含量、血清Ca2+、P水平变化；采用ELISA法检测血清中TNF-α、IL-6、IL-1β含量；采用qRT-PCR法检测骨组织中的TLR4、MyD88、NF-κBmRNA表达水平；采用Western blot法检测骨组织TLR4、MyD88、NF-κB蛋白表达水平；采用骨组织转录组学检测TLR4、MyD88、NF-κB 表达水平。结果 (1) 补肾活血汤组中大鼠骨组织骨矿含量以及血清Ca2+ 、P水平较模型组均升高（P＜0.05）；（2）补肾活血汤组大鼠股骨和腰椎BMD含量较模型组均明显升高（P＜0.01）；（3）与模型组比较，补肾活血汤组血清IL-6、IL-1β、TNF-a含量降低（P＜0.05）；（4）与模型组比较，补肾活血汤组TLR4、MyD88、NF-κBmRNA表达量降低（P＜0.05）；(5)与模型组比较，补肾活血汤组TLR4、MyD88、NF-κB蛋白表达量降低（P＜0.05）；（6）骨组织转录组学发现补肾活血汤组TLR4、MyD88、NF-κB表达量降低（P＜0.05）。结论补肾活血汤能够通过抑制TLR4/MyD88/NF-κB信号轴来防治骨质疏松症。

英文摘要:

Objective To explore the effect of reinforcing kidney and activating blood (RKAB) decoction on osteoporosis (OP) based on TLR4/Myd88/NF-κB signal axis. Methods Forty SD rats were randomly divided into sham operation group, model group, RKAB decoction group, and alendronate sodium group, with 10 rats in each group. Rats in the RKAB decoction group received RKAB decoction 10 mL/kg/d by gavage. Rats in the alendronate sodium group received alendronate sodium 7.35 mg/kg/d by gavage. Rats in the sham operation group and the model group received the same amount of normal saline by gavage. After 12 weeks of intervention, bone mineral density (BMD), bone mineral content (BMC), and serum Ca2+ and P levels were measured. Serum levels of TNF-α, IL 6, and IL 1β were measured with ELISA. The mRNA expression levels of TLR4, Myd88, and NF-κB in the bone tissue were detected with qRT-PCR. The protein expression levels of TLR4, Myd88, and NF-κB in the bone tissue were detected with Western blotting. The expression levels of TLR4, MyD88, and NF-κB were detected using bone transcriptomics. Results (1) BMC in the bone tissue and the levels of serum Ca2+ and P in the RKAB decoction group were higher than those in the model group (P<0.05). (2) BMD of the femur and lumbar vertebrae in the RKAB decoction group were higher than those in the model group. (3) Compared to those in the model group, the levels of IL-6, il-1β, and TNF-a in the RKAB decoction group were lower (P<0.05). (4) Compared to those in the model group, the levels of IL-6, il-1β, and TNF-a in the RKAB decoction group were lower (P<0.05). (5) Compared to those in the model group, the expression of TLR4, Myd88, and NF-κB in the RKAB decoction group decreased significantly (P<0.05), and the expression of TLR4, Myd88, and NF-κB in the RKAB decoction group decreased significantly (P<0.05). (6) The expression levels of TLR4, Myd88, and NF-κB in the RKAB decoction group were significantly lower than those in control group (P<0.05). Conclusion RKAB decoction prevents and treats osteoporosis by inhibiting TLR4/Myd88/NF-ΚB signal axis.

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