| Objective Research on the main mechanism of Xiangsha Liujunzi Decoction in preventing and treating postmenopausal osteoporosis. Methods SD female rats were randomly divided into 6 groups: blank group, model group, positive control group (Xian Ling Gu Bao capsule group), low, medium and high dose groups of Xiangsha Liujunji Decoction. Ovariectomy (OVX) method was used to make the model. After the model was successfully made, Chinese medicine was used to intervene. Materials were taken eight weeks later. MicroCT was used to detect bone tissue structure, biomechanical instrument was used to detect the maximum load of femur, ELISA was used to detect bone metabolism, and immunohistochemistry (IHC) was used to detect bone formation related proteins and pathway proteins. Results Compared with the model group, Xiangsha Liujunzi Decoction in each dose group could restore bone microstructure (P<0.05), especially in the high-dose group, so we used the high-dose group as the drug for this experiment; It also could significantly increase the maximum load on the femur of rats (P<0.05); Significantly increase and decrease the content of ALP and PINP in serum β-CTX content level (P<0.01); Effectively increase the percentage of bone trabeculae (P<0.05); Promote the expression of Runx 2, the key protein regulating bone formation, and the osteogenic pathway related protein Wnt and β- Catenin expression (P<0.05). Conclusion Xiangsha Liujunzi Decoction can restore the continuity and stability of the femoral trabecular bone of OVX rats, promote bone formation, improve bone density, and thus prevent and treat PMOP, and its mechanism may be related to the activation of Wnt/ β- Catenin signaling pathway up-regulates the expression of Runx2, which contributes to bone differentiation. |