中国骨质疏松杂志

电针联合延胡索水提物对绝经后骨质疏松大鼠的改善作用

Improvement of postmenopausal osteoporosis in rats by electroacupuncture combined with aqueous extracts of Yanhusuo

DOI：10.3969/j.issn.1006-7108.2023.08.011

英文关键词:osteoporosis electroacupuncture water extract of corydalis Yanhusuo combination of acupuncture and medicine bone metabolism

基金项目:河北省中医药管理局课题（2023031）

作者	单位
温志刚1 路帅2 马欣3*	1. 河北省中医院骨伤二科，河北石家庄 050000 2. 石家庄市中医院，河北石家庄 050000

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中文摘要:

目的探究电针联合延胡索水提物对骨质疏松症大鼠的保护作用，并初步探讨其可能的潜在机制。方法采用双侧卵巢切除法制备骨质疏松症大鼠模型，根据处理方式分为Sham组(假手术组)、模型组、雌二醇组、电针组、延胡索组和针药组。采用苏木精-伊红染色检测骨组织形态并测量骨小梁结构参数；采用酶联免疫吸附测定检测血清骨代谢标志物、骨保护素（OPG）和核因子-κB配体的受体激活剂（RANKL）的含量；采用蛋白免疫印迹检测骨组织中Runx2、Wnt3a和β-catenin蛋白的表达。结果治疗后，与Sham组相比，模型组大鼠的骨密度值明显降低，而骨代谢标志物水平明显上升（P＜0.05）；电针、延胡索提取物及二者联合治疗能够减轻模型组大鼠的骨小梁结构和骨组织形态损伤，并降低骨代谢标志物含量（P＜0.05）；电针、延胡索水提物及二者联合治疗能够降低OPG含量、抑制Runx2、Wnt3a和β-catenin的蛋白表达，且增加RANKL含量以及GSK-3β和DVL1蛋白表达（P＜0.05）；电针与延胡索水提物联合干预的效果明显优于电针和延胡索水提物单独使用的效果（P＜0.05）。结论电针刺激与延胡索水提物联合使用能够起到协同增效作用，通过调控OPG/RANKL和Wnt/β-catenin途径来减少骨量损失、增加骨密度、改善骨组织的微观结构和骨代谢，从而改善卵巢切除术诱导的骨质疏松症。

英文摘要:

Objective The protective effect of electroacupuncture combined with aqueous extract of Yanhusuo on osteoporotic rats was investigated, and its possible potential mechanism was tentatively explored. Methods A bilateral ovariectomy method was used to prepare an osteoporotic rat model, which was divided into: Sham group, model group, estradiol group, electroacupuncture group, Yanhusuo group and needle medicine group according to the treatment. Hematoxylin-eosin staining was used to detect bone histomorphology and measure structural parameters of bone trabeculae; Enzyme-linked immunosorbent assay was used to detect serum bone metabolic markers, osteoprotegerin (OPG) and receptor activator of nuclear factor -κB ligands (RANKL); Western blotting was used to detect the expression of Runx2, Wnt3a and β-catenin proteins in bone tissue. Results After treatment, the BMD values of rats in the model group were significantly lower and the levels of bone metabolic markers were significantly higher in the model group compared with the Sham group (P<0.05). Electroacupuncture, Yanhusuo extract and the combination of both treatments were able to reduce the structural and morphological damage of bone trabeculae and bone metabolic marker levels in the model group rats (P<0.05). The combined treatment of electroacupuncture, aqueous extract of Yanhusuo and both of them could inhibit the reduction of OPG content, inhibit the protein expression of Runx2, Wnt3a and β-catenin, and increase the content of RANKL and the protein expression of GSK-3β and DVL1. The effect of combined intervention of electroacupuncture and aqueous extract of Yanhusuo was significantly better than that of electroacupuncture and aqueous extract of Yanhusuo alone (P<0.05). Conclusion The combination of electroacupuncture stimulation and Yanhusuo aqueous extract can play a synergistic role in reducing bone loss, increasing bone density, improving bone tissue microstructure and bone metabolism by regulating OPG/RANKL and Wnt/ β-catenin pathways, thus improving ovariectomy-induced osteoporosis.

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