| Objective To study the regulatory effect of long non-coding RNA (lncRNA) differentiation antagonizing non-protein coding RNA (DANCR) targeting microRNA (miR)-19a-3p on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in postmenopausal osteoporosis (PMOP). Methods BMSCs were isolated from 4 healthy female volunteers and 4 PMOP patients and cultured. BMSCs of PMOP patients were grouped and transfected with negative control (NC) siRNA, lncRNA DANCR siRNA, miR-NC inhibitor, or miR-19a-3p inhibitor. The expression levels of lncRNA DANCR, miR-19a-3p, and PTEN were measured. OD 405 levels, vitality of alkaline phosphatase (ALP), mRNA expression levels of osteocalcin (OCN) and osteopontin (OPN) after osteogenic induction and differentiation were measured. Results The expression levels of lncRNA DANCR and PTEN in BMSCs of PMOP patients were higher than those in healthy women. The expression level of miR-19a-3p and OD 405 level, ALP viability, OCN and OPN expression levels after osteogenic induction were lower than those in healthy women. In BMSCs of PMOP patients, the expression levels of lncRNA DANCR and PTEN in the si-DANCR group were lower than those in the si-NC group, and the OD 405 levels, ALP viability, and the expression levels of miR-19a-3p, OCN, and OPN were higher than those in the si-NC group. The levels of OD 405 level and ALP viability and the expression levels of miR-19a-3p, OC, and OPN in si-DANCR+miR-19a-3p inhibitor group were lower than those of the si-DANCR+miR-NC inhibitor group. The expression level of PTEN was higher than that in the si-DANCR+miR-NC inhibitor group. Conclusion LncRNA DANCR targeting miR-19a-3p regulates the osteogenic differentiation of BMSCs in PMOP patients. |