| Objective To explore the promoting effect of overexpression of miR-664a-5p in ADSCs (miR-664a-5p-ADSCs) on bone remodeling in osteoporosis (OP) rats via activating Wnt/β-catenin signal pathway. Methods Rats were divided into Sham group, OVX group, ADSCs group, and miR-664a-5p-ADSCs group. OP rat model was established by ovariectomy. Adipose tissue was removed only in Sham group. After 3 months, rats in the ADSCs group and the miR-664a-5p-ADSCs group were injected with ADSCs or miR-664a-5p-ADSCs through tail vein, respectively. Rats in Sham group and OVX group were injected with the same amount of normal saline. Index detection was performed after 1 month. Micro-CT, HE staining, and TRAP staining were used to detect bone remodeling in OP rats. Immunohistochemical staining, RT-qPCR, and Western blotting were used to detect the expressions of Runx2, ALP, OCN, Wnt, and β-Catenin mRNA and protein. Results In Sham group, the bone trabeculae were complete and osteoclasts were few. Compared with that in Sham group, the bone trabeculae in OVX group were sparse and thin, and there were more osteoclasts. The levels of Runx2, ALP, OCN, Wnt, and β-Catenin mRNA and protein decreased significantly (P<0.05). Compared with those in OVX group, bone trabeculae in ADSCs group and miR-664a-5p-ADSCs group increased and thickened, and osteoclasts were less. The levels of Runx2, ALP, OCN, Wnt, and β-Catenin mRNA and protein increased significantly (P<0.05). The above effects in miR-664a-5p-ADSCs group were stronger than those in ADSCs group (P<0.05). Conclusion Overexpression of miR-664a-5p in ADSCs promotes bone remodeling in OP rats via activating Wnt/β-catenin signal pathway. |