中国骨质疏松杂志

石斛多糖对胶原诱导关节炎小鼠免疫活性的影响

Effect of Dendrobium sonia polysaccharide on immune activity of collagen-induced arthritis mice

DOI：10.3969/j.issn.1006-7108.2023.10.013

英文关键词:dendrobium sonia polysaccharide collagen-induced arthritis Th17/Treg cell ratio inflammation

基金项目:武汉市卫生健康委基金项目（WZ20C15）；全国名老中医药专家传承工作室建设项目（国中医药人教发〔2016〕42号）；武汉市卫生健康委基金项目（WZ21C28）

作者	单位
阮崇洁李勇[]* 黄裳	武汉市中医医院针灸风湿病科，湖北武汉 430014

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中文摘要:

目的探究石斛多糖（DSP）对胶原蛋白诱导关节炎（CIA）小鼠体内辅助T（Th17）/调节性T（Treg）细胞比例平衡及免疫炎症的影响。方法构建CIA小鼠，通过关节HE染色和后肢右爪形态观察进行模型鉴定。分别给予CIA小鼠100、200和300 mg/kg DSP灌胃处理将小鼠分为对照组，模型组，低、中、高浓度DSP组。观察小鼠足爪病变情况，HE染色观察小鼠右肢滑膜组织病理改变，流式细胞术检测小鼠脾脏中Th17细胞，Treg细胞比例和CD4+CD28?PD-1lowOX40+T细胞比例的变化，免疫组化法观察小鼠右肢膝关节中IL-7的表达水平，ELISA检测不同时间点（0、14、28、42 d）小鼠血清IL-6、IL-10及小鼠右肢膝关节IL-6和IL-10表达水平。结果与对照组小鼠相比，模型组小鼠足爪病变加重，滑膜组织病理变化明显，Th17细胞比例上升，Treg细胞和CD4+CD28?PD-1lowOX40+T细胞比例下降，IL-7阳性表达增加，Foxp3阳性表达下降，血清和膝关节中IL-6水平增加，IL-10水平下降（均P<0.0.5）。与模型组相比，低、中、高浓度DSP组小鼠足爪病变减轻，滑膜组织病理变化改善，Th17细胞比例下降，Treg细胞和CD4+CD28?PD-1lowOX40+T细胞比例上升，IL-7阳性表达减少，Foxp3阳性表达增多，血清和膝关节中IL-6水平下降，IL-10水平增加（均P<0.0.5），且高浓度DSP作用效果最好。结论 DSP对CIA小鼠体内Th17/Treg细胞比例平衡具有调节作用，能抑制炎症反应，减轻病理改变。

英文摘要:

Objective To investigate the effects of Dendrobium polysaccharide (DSP) on helper T (Th17)/regulatory T (Treg) cell balance and immune inflammation in collagen-induced arthritis (CIA) mice. Methods CIA mice were induced, and the model was identified by joint HE staining and morphological observation of the right hind paw. CIA mice were given 100, 200 and 300 mg/kg DSP intragastric treatment and divided into control group, model group, low DSP group, medium DSP group and high DSP group. HE staining was used to observe the pathological changes of the synovium of the right limb of mice in each group. Flow cytometry was used to detect the changes of Th17 cells, Treg cells and CD4+CD28-PD-1lowOX40+ T cells in spleen of mice in each group. The expression levels of IL-7 in the right knee joint of mice in each group were observed by immunohistochemistry, and the expression levels of IL-6 and IL-10 in serum and IL-6 and IL-10 in the right knee joint of mice in each group were detected by ELISA at different time points (0, 14, 28, 42 d). Results Compared with control group, the paw lesions of model group were aggravated; the synovial tissue pathological changes were obvious; the proportion of Th17 cells was increased; the positive expression of Foxp3 was decreased; the proportion of Treg cells and CD4+CD28-PD-1lowOX40+ T cells was decreased; the positive expression of IL-7 was increased, and the content of IL-6 was increased, IL-10 content decreased in serum and knee joint (all P<0.0.5). Compared with model group, the paw lesions of mice in low, medium and high concentration DSP groups were reduced; the synovial tissue pathological changes were improved; the proportion of Th17 cells decreased, the proportion of Treg cells and CD4+CD28-PD-1lowOX40+T cells increased, the positive expression of IL-7 decreased, the positive expression of Foxp3 increased.And the content of IL-6 decreased, IL-10 content increased in serum and knee joint (all P<0.0.5), and high concentration of DSP had the best effect. Conclusion DSP can regulate the balance of Th17/Treg cells in CIA mice, inhibit inflammation and alleviate pathological changes.

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