Objective To detect and analyze transcription factor interacting with the upstream promoter region of CLCF1 gene. Methods DNA pulldown and mass spectrometry were used to detect the proteins interacting with the promoter region of CLCF1 gene. The identified proteins were analyzed using bioinformatics. Fifty candidate transcription factors were screened out. Parallel reaction monitoring (PRM) was used to verify the expression of the transcription factors. Results Two hundred and fifty-one proteins were screened in the experimental group and the control group. GO enrichment analysis showed that they were mainly involved in ribosome biogenesis, positive regulation of DNA-templated transcription, transcription regulator complex, SWI/SNF superfamily-type complex, cAMP response element binding, and ATP hydrolysis activity. KEGG pathway showed that these differential proteins were mainly involved in human T-cell leukemia virus 1 infection, mitophagy, and thyroid hormone signaling pathway. PRM verified thtat the expression trends of MAFK, TFE3, MITF, JUNB, and FOSL2 were consistent with the DNA pulldown detection results. Conclusion MAFK, TFE3, MITF, JUNB, and FOSL2 may bind to the promoter region of CLCF1 gene. |