|
| 葛根素调控SLC7A11/GPX4轴抑制高糖诱导的成骨细胞铁死亡 |
| Puerarin inhibits high glucose-induced ferroptosis in osteoblasts by modulation of the SLC7A11/GPX4 axis |
| |
| DOI：10.3969/j.issn.1006-7108.2023.12.015 |
| 中文关键词: 糖尿病性骨质疏松 葛根素 成骨细胞 铁死亡 SLC7A11/GPX4轴 |
| 英文关键词:diabetic osteoporosis puerarin osteoblasts ferroptosis SLC7A11/GPX4 axis |
| 基金项目: |
|
| 摘要点击次数: 1647 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 探讨葛根素对高糖环境下成骨细胞铁死亡的保护作用及其分子机制。方法 用高糖培养基诱导MC3T3-E1细胞铁死亡，使用不同浓度葛根素进行干预。通过CCK-8检测葛根素对MC3T3-E1细胞活性的影响；通过ALP染色、ARS染色、qRT-PCR检测葛根素对MC3T3-E1细胞成骨分化能力的影响；通过流式细胞技术、DHE染色和试剂盒测定MC3T3-E1骨细胞中ROS、GSH、SOD和MDA水平；通过Western blot检测MC3T3-E1细胞中SLC7A11和GPX4的表达情况。结果 CCK-8结果表明，100 μmol/L以下浓度的葛根素对MC3T3-E1细胞活性无明显抑制作用；与高糖组相比，100 μmol/L的葛根素能够有效减轻高糖对MC3T3-E1细胞增殖的抑制作用（P＜0.05）；与高糖组相比，葛根素干预后能明显提高ALP、RUNX2、COL1A1、OPN的基因表达，增加MC3T3-E1细胞ALP活性和矿化水平（P＜0.05）；与高糖组相比，葛根素干预后明显降低了MC3T3-E1细胞中ROS、MDA水平，增加了GSH、SOD活性（P＜0.05）；Western blot结果表明，高糖干预明显降低了MC3T3-E1细胞中SLC7A11和GPX4的表达，葛根素干预后上调了SLC7A11和GPX4的表达（P＜0.05）。结论 葛根素能够减轻高糖诱导的成骨细胞铁死亡，其作用机制可能是通过调控SLC7A11/GPX4信号通路实现的。 |
| 英文摘要: |
| Objective To investigate the protective effect of puerarin against ferroptosis in high glucose-induced ferroptosis in osteoblasts and its molecular mechanism. Methods Ferroptosis in MC3T3-E1 osteoblasts was induced by high glucose medium, using different concentrations of puerarin for the intervention. The effect of puerarin on the activity of MC3T3-E1 cells were detected with CCK-8. The effect of puerarin on the osteogenic differentiation ability of MC3T3-E1 cells were detected using ALP staining, ARS staining, and qRT-PCR. The levels of ROS, GSH, SOD, and MDA in MC3T3-E1 were measured with flow cytometry, DHE staining, and kits. The expressions of SLC7A11 and GPX4 were detected with Western blotting. Results CCK-8 results showed that puerarin at concentrations below 100 μM had no significant inhibitory effect on MC3T3-E1 cell activity. Compared with that in the high glucose group, 100 μM of puerarin effectively attenuated the inhibitory effect of high glucose on proliferation of MC3T3-E1 cells (P<0.05). In addition, puerarin significantly increased the expressions of ALP, RUNX2 COL1A1, and OPN, and increased ALP activity and mineralization level in MC3T3-E1 cells compared to those in the high glucose group (P<0.05). Compared with the high glucose group, puerarin markedly decreased ROS and MDA levels and increased GSH and SOD activities in MC3T3-E1 cells (P<0.05). Western blotting results showed that high glucose intervention obviously decreased the expressions of SLC7A11 and GPX4 in MC3T3-E1 cells, whereas puerarin upregulated the expressions of SLC7A11 and GPX4 (P<0.05). Conclusion Puerarin is able to attenuate high glucose-induced ferroptosis in osteoblasts. Its mechanism may be achieved by regulating the SLC7A11/GPX4 signaling pathway. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|