| Objective To investigate the changes of asprosin level in patients with type 2 diabetes mellitus complicated with osteoporosis (T2DOP) and its relationship with toll-like receptor 4/ c-Jun N-terminal protein kainse/interleukin1β (TLR4/JNK/IL-1β) inflammatory signaling pathway. Methods A total of 123 T2DM patients admitted to Huanggang Central Hospital from January 2020 to January 2023 were selected and divided into three groups according to the T value calculated based on bone mineral density (BMD). Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of serum asprosin, osteocalcin (OC), bone-derived alkaline phosphatase (BALP), type Ⅰ procollagen N-terminal propeptide (P1NP), TLR4, p-JNK/JNK, and IL-1β in the three groups. Pearson correlation coefficient was used to analyze the correlation between asprosin and TLR4, p-JNK /JNK, IL-1β, BMD, OC, and BALP. Multivariate logistic regression was used to analyze the risk of asprosin for T2DOP. Receiver operating characteristic (ROC) curve was used to determine the diagnostic value of asprosin for T2DOP. Results There were significant differences in serum asprosin levels among the three groups (P<0.05). Serum asprosin level was positively correlated with TLR4, p-JNK/JNK, and IL-1β (P<0.05), but negatively correlated with BMD, OC, and BALP (P<0.001). Multivariate logistic regression analysis showed that increased serum asprosin level was a risk factor for inducing T2DOP (P<0.05). ROC curve analysis showed that serum asprosin cut-off value of 230.5 ng/mL had good sensitivity (80.43%) and specificity (85.00%) for T2DOP prediction. Area under the curve, AUC, was 0.844 (95% CI: 0.818-0.959, P<0.001). Conclusion Serum asprosin level is significantly increased in T2DOP patients, and is negatively correlated with BMD, OC, and BALP. Asprosin level is a risk factor for T2DOP. It has good diagnostic value in predicting T2DOP. It may promote the occurrence and development of T2DOP by activating the TLR4/JNK/IL-1β signaling pathway. |