| Objective To explore the effects of platelet-rich plasma combined with sodium hyaluronate on bone healing, angiogenesis and NFATc1 signaling pathway in rats with osteoporotic fractures. Methods Fifty SPF SD female rats were randomly divided into normal (NO) group, model (MO) group, platelet-rich plasma (PP) group, sodium hyaluronate (SH) group, and platelet-rich plasma combined with sodium hyaluronate (PS) group. Bone mineral density was detected by bone densitometry in small animals, bone biomechanics was detected by biomechanics testing machine, bone tissue pathological morphology was detected by HE staining, serum angiogenesis related factors were detected by ELISA, and NFATc1 signaling pathway protein expression in rat bone tissue was detected by western blot. Results Compared with NO group, bone mineral density, stiffness, maximum load, maximum pressure, elastic modulus, number and area of blood vessels, VEGF, bFGF and ANG-1 contents in MO were significantly decreased (P<0.05), while the expressions of NFATc1 and OSCAR protein in femur tissue were significantly increased (P<0.05). Bone mineral density, stiffness, maximum load, maximum pressure, elastic modulus, blood vessel number and blood vessel area, VEGF, bFGF and ANG-1 contents in PP, SH and PS groups were significantly increased (P<0.05), while the expressions of NFATc1 and OSCAR proteins in femur tissue were significantly decreased (P<0.05). There was no significant difference between SH group and PP group (P>0.05), and PS group had significant change compared with SH group (P<0.05). Conclusion Platelet-rich plasma combined with sodium hyaluronate has a significant effect on osteoporotic fracture rats, which can effectively promote bone healing and angiogenesis, and inhibit NFATc1 signaling pathway. |