| Objective To evaluate the effects of salidroside and specnuezhenide on reversing iron overload, promoting bone formation, and anti postmenopausal osteoporosis (PMOP), and to explore the interaction between them. Methods Zebrafish osteoporosis model induced by ferric ammonium citrate was established to evaluate the effects of salidroside (1, 5, 25, 50, 100 μmol/L), specnuezhenide (1, 5, 25, 50, 100 μmol/L) and their mixed solutions (1:1) on bone mineralization. After Alizarin red staining, bone mineralization was evaluated with bone staining area and cumulative optical density. The interaction between salidroside and specnuezhenide was evaluated with Isobole method and Isobolographic analysis method. Results Salidroside, specnuezhenide, and their mixed solution reversed the inhibition of bone formation caused by iron overload, and showed a significant concentration dependence in the range of 1-100 μM. The half effective concentration (EC50) γ > 1 calculated by with the Isobole method, indicating that there was no obvious synergy. The sum of the EC50 ratio scores of the corresponding components in the mixed group was 1.16 according to the isobolographic analysis method, and its composite point fell within the 95% confidence limit (P>0.05), indicating that there was an additive relationship and there was no obvious synergistic effect. Conclusion Salidroside and specnuezhenide reverse the inhibition of bone formation caused by iron overload. The combination show additive effect. |