| Objective To explore the correlation between serum hepcidin concentration and bone density in the population, and to understand the effect of hepcidin on the differentiation of mouse osteoblast precursor cells (MC3T3-E1) and its related mechanisms. Method ① Enzyme linked immunosorbent assay (ELISA) was used to detect serum hepcidin levels in two groups of people with normal bone mass and osteoporosis;② Using cell counting reagents to detect and compare the effects of osteogenic precursor cells (MC3T3-E1) on cell proliferation after intervention with different concentrations of hepcidin RT-PCR and Western Blot methods were used to investigate the effects of hepcidin on osteogenic differentiation functional indicators (ALP, Runx2, BMP2, P-SMAD1/5, and SMAD5). Result There was a significant positive correlation between serum hepcidin levels and bone mineral density of the lumbar spine and hip in the population; An increase in the concentration of hepcidin within a certain range (0-10 ng/mL) can significantly upregulate the expression of cell functional differentiation (ALP, Runx2) and promote the expression of osteogenic signaling pathway proteins (BMP2, P-SMAD1/5, and SMAD5). Conclusion Clinical data shows a significant correlation between serum hepcidin levels and bone mass, with low hepcidin levels and low bone density values; Cell experiments have shown that a certain concentration of hepcidin can significantly activate the BMP2-SMAD1/5 signaling pathway, upregulate the expression of osteogenic differentiation related genes, and induce cell osteogenic differentiation; Therefore, this study suggests that serum hepcidin may be correlated with osteoporosis, and clinical detection of hepcidin may be a new evaluation indicator for the diagnosis and treatment of osteoporosis, which is worth further research. |