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| 针刺脾俞对衰老大鼠腰骨骼肌萎缩Akt/NF-κB信号通路的影响 |
| Study on the prevention of back pain caused by skeletal muscle atrophy in aging rats by acupuncture of Pi Shu |
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| DOI：10.3969/j.issn.1006-7108.2024.04.003 |
| 中文关键词: 衰老性骨骼肌萎缩 针刺 脾俞 Akt/NF-κB信号通路 |
| 英文关键词:senile skeletal muscle atrophy acupuncture Pi Shu Akt/NF-κB signaling pathway |
| 基金项目:福建省中青年教师教育科研项目（JAT190260）；福建中医药大学校管项目（XZJ2022004） |
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| 中文摘要: |
| 目的 观察针刺脾俞对衰老大鼠Akt/NF-κB信号通路的影响，探讨针刺脾俞改善骨骼肌萎缩以防治腰痛的可能机制。方法 将32只雄性SD大鼠随机分为空白组、模型组、针刺组、阳性药物组，每组8只。空白组大鼠每日项背部皮下注射0.9 %生理盐水 [300 mg/(kgd)]，其余3组大鼠每日项背部皮下注射等量的D-半乳糖，连续12周。第9周开始，针刺组取双侧脾俞进行针刺，阳性药物组采用混合氨基酸灌胃，连续4周。用HE染色对骨骼肌组织形态进行观察，TdT-mediated dUTP nick-end labeling（TUNEL）法检测骨骼肌细胞凋亡情况， ELISA检测IL-1β、IL-6T、NF-α释放量，Western Blot检测Akt、p-Akt 、NF-κB、p-NF-κB 的蛋白表达水平。结果 与空白组比较，模型组骨骼肌细胞间隙变宽，部分肌细胞圆形化，肌细胞凋亡数量增多，炎性因子IL-1β、IL-6T、NF-α水平显著上升（P＜0.001、P＜0.01和P＜0.05）, p-Akt、NF-κB、p-NF-κB蛋白表达水平升高（P＜0.001）；与模型组比较，针刺组和阳性药物组，肌间隙窄，肌细胞排列更紧密，凋亡数量减少，炎性因子IL-1β、IL-6T、NF-α水平下降（P＜0.01、P＜0.01和P＜0.05），p-Akt、NF-κB、p-NF-κB蛋白表达水平下调（P＜0.001）。结论 针刺脾俞可改善D-半乳糖诱导的骨骼肌衰老，并通过抑制Akt/NF-κB信号通路，降低炎症反应，减少骨骼肌细胞凋亡，这可能是针刺脾俞治疗腰痛的潜在作用机制之一。 |
| 英文摘要: |
| Objective To observe the effect of acupuncture on the Akt/NF-κB signaling pathway in aging rats, and to explore the possible mechanism of acupuncture on the Pi Shu point to improve skeletal muscle atrophy to prevent and treat low back pain. Methods 32 male SD rats were randomly divided into blank group, model group, acupuncture group and positive drug group, 8 rats in each group. The rats in the blank group were injected subcutaneously with 0.9% saline[300 mg/(kgd)] on the back of the collar daily, and the rats in the remaining three groups were injected subcutaneously with an equal amount of D-galactose on the back of the collar daily for 12 weeks. From the 9th week, the acupuncture group was treated with acupuncture on both sides of the Pi Shu, while the positive drug group was treated with mixed amino acid gavage for 4 weeks. The skeletal muscle tissue morphology was observed by HE staining, the apoptosis of skeletal muscle cells was detected by TdT-mediated dUTP Nick-End Labeling（TUNEL）, the release of TNF-α, IL-1β and IL-6 was detected by Elisa, and the protein expression levels of Akt, p-Akt, NF-κB and p-NF-κB were detected by Western Blot. Results Compared with the blank group, the skeletal muscle cell gap in the model group became wider, some myocytes were rounded, the number of myocyte apoptosis was high, and the levels of inflammatory factors IL-1β , IL-6 and TNF-α were significantly increased (P < 0.001, P < 0.01 and P < 0.05), and the protein expression levels of p-Akt , NF-κB and p-NF-κB were increased (P < 0.001); Compared with the model group, the muscle gap in the acupuncture and positive drug groud was narrower, myocytes were more closely arranged, the number of myocyte apoptosis was reduced, the levels of inflammatory factors IL-1β , IL-6andTNF-α decreased (P < 0.01, P < 0.01 and P < 0.05), and the expression levels of p-Akt, NF-κB and p-NF-κB were down-regulated (P < 0.001). Conclusions Acupuncture at Pi Shu can improve D-galactose-induced skeletal muscle aging and reduce the inflammatory response and apoptosis of skeletal muscle cells by inhibiting the Akt/NF-κB signaling pathway, which may be one of the potential mechanisms of action of acupuncture at Pi Shu point for the treatment of low back pain. |
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