|
| 马钱子苷通过肠道菌群和神经肽抑制OVX小鼠骨量丢失的机制探讨 |
| Explore the mechanism of Loganin in prevention of bone loss in OVX mice via gut microbiota and neuropeptides |
| |
| DOI：10.3969/j.issn.1006-7108.2024.04.012 |
| 中文关键词: 中医中药 马钱子苷 去卵巢小鼠 骨质疏松 |
| 英文关键词:traditional Chinese medicine loganin ovariectomized mice osteoporosis |
| 基金项目:广东省云浮市科技计划项目（2020A090402）；国家自然科学基金项目（青年科学基金）（81704098） |
|
| 摘要点击次数: 27 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 观察马钱子苷（Loganin）干预去卵巢（OVX）小鼠骨量、肠道菌群及神经肽的变化。方法 28只雌性C57BL/6J小鼠随机分为假手术组（SHAM）、OVX组、Loganin低剂量组（Loganin-L）和Loganin高剂量组（Loganin-H）,每组7只。Loganin-L组和Loganin-H组在OVX基础上分别予Loganin 5 mg/(kg·d)和20 mg/(kg·d)灌胃，SHAM组和OVX组灌胃等量蒸馏水，每日1次，持续12周。实验结束后处死取材，Micro-CT检测左侧股骨BMD、BV/TV、Tb.N及Tb.Sp；ELISA法检测血清中CTX、TRAP、P1NP、TNF-α、IL-6、IL-10、LPS、CGRP、SP、VIP、NPY水平；16S rRNA高通量测序技术检测粪便中肠道菌群多样性与差异性。结果 OVX组BMD、BV/TV、Tb.N、P1NP、IL-10、CGRP、SP、VIP、NPY较SHAM组显著降低（P<0.05，P<0.01），Tb.Sp、CTX、TRAP、TNF-α、IL-6、LPS显著增高（P<0.05，P<0.01）。Loganin-L组BMD、BV/TV、Tb.N、P1NP、IL-10、CGRP、SP、VIP、NPY较OVX组有升高趋势（P>0.05），Tb.Sp、CTX、TRAP、TNF-α、IL-6、LPS有降低趋势（P>0.05）。Loganin-H组BMD、BV/TV、Tb.N、P1NP、IL-10、CGRP、SP、VIP、NPY较OVX组显著增高（P<0.05），Tb.Sp、CTX、TRAP、TNF-α、IL-6、LPS显著降低（P<0.05）。在门水平，与SHAM组相比，OVX组Firmicutes/Bacteroidota(F/B)率显著降低（P<0.05），Loganin-L组F/B率较OVX组有升高趋势（P>0.05），Loganin-H组F/B率显著升高（P<0.05）；在属水平，OVX组norank_f__Muribaculaceae丰度较SHAM组显著增加（P<0.05），Lactobacillus丰度显著减少（P<0.05）；较OVX组，Loganin-L组norank_f__Muribaculaceae丰度有降低趋势（P>0.05），Lactobacillus丰度有升高趋势（P>0.05），Loganin-H组norank_f__Muribaculaceae丰度显著减少（P<0.05），Lactobacillus丰度显著增加（P<0.01）。结论 Loganin呈剂量依赖性抑制OVX小鼠骨量丢失，其机制可能是通过调节肠道菌群中norank_f__Muribaculaceae和Lactobacillus丰度、增加神经肽含量及降低炎症反应实现的。 |
| 英文摘要: |
| Objective To observe the changes of bone mass, gut microbiota and neuropeptides in ovariectomized mice treated with Loganin. Methods 28 female C57BL/6J mice were randomly divided into sham operation group (SHAM), ovariectomized group (OVX), Loganin low-dose group (Loganin-L）and Loganin high-dose group (Loganin-H), with 7 mice in each group. Loganin-L and Loganin-H groups received Loganin 5 mg/(kg·d) and 20 mg/(kg·d) via oral gavage, respectively, while SHAM and OVX groups received the same dose of distilled water via oral gavage. After 12 weeks of intervention, BMD, BV/TV, Tb.N and Tb.Sp of the left femur were detected by Micro-CT. CTX, TRAP, P1NP, TNF-α, IL-6, IL-10, CGRP, SP, VIP, NPY and LPS were detected by ELISA. 16S rRNA high-throughput sequencing was used to detect the gut microbiota. Results BMD, BV/TV, Tb.N, P1NP, IL-10, CGRP, SP, VIP and NPY were significantly decreased in OVX group compared with SHAM group (P<0.05, P<0.01); Tb.Sp, CTX, TRAP, TNF-α, IL-6 and LPS were significantly increased in OVX group (P<0.05, P<0.01). BMD, BV/TV, Tb.N, P1NP, IL-10, CGRP, SP, VIP, and NPY of Loganin-L group tended to increase compared with OVX group (P>0.05), while Tb.Sp, CTX, TRAP, TNF-α, IL-6 and LPS tended to decrease (P>0.05). BMD,BV/TV, Tb.N, P1NP, IL-10, CGRP, SP, VIP and NPY were significantly increased in Loganin-H group compared with OVX group (P<0.05); Tb.Sp, CTX, TRAP, TNF-α, IL-6 and LPS were significantly decreased (P<0.05). At the phylum level, F/B ratio was significantly decreased in OVX group (P<0.05) compared with SHAM group. However the F/B ratio in Loganin-L group has an increasing trend compared with OVX group (P>0.05), and it was significantly increased in Loganin-H group (P<0.05). At the genus level, norank_f_ Muribacula ceae exhibited a significant increase (P<0.05) while Lactobacillus demonstrated a significant decrease (P<0.05) in OVX group compared with SHAM group. Compared with OVX group, norank_f__Muribaculaceae tended to decrease in Loganin-L group(P>0.05), and Lactobacillustended to increase (P>0.05). Norank_f__Muribaculaceae showed a significant decrease (P<0.05) whereas Lactobacillus exhibited a significant increase (P<0.01) in Loganin-H group. Conclusion Loganin can inhibit bone loss in OVX mice in a dose-dependent manner, which may be achieved by reducing inflammatory response, increasing the level of neuropeptides and regulating the abundance of norank_f__Muribaculaceae and Lactobacillus. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|