跑台运动对切除卵巢大鼠骨质疏松症的保护机制
The protective mechanism of treadmill exercise on osteoporosis in ovariectomized rats
  
DOI:10.3969/j.issn.1006-7108.2024.06.008
中文关键词:  跑台运动  绝经后骨质疏松症  Hedgehog-Gli信号通路  大鼠
英文关键词:treadmill exercise  postmenopausal osteoporosis  Hedgehog-Gli signaling pathway  rat
基金项目:江西省教育厅科技项目课题(GJJ209916)
作者单位
张帅军1* 张锦2 杜旭辉2 黄兴裕4 牛英鹏3 1.赣南师范大学科技学院江西 赣州 341000 2.平顶山市第一人民医院河南 平顶山 467000 3.河南大学河南 开封 475001 4.赣南师范大学江西 赣州 341000 
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中文摘要:
      目的 由Hedgehog-Gli信号通路探讨跑台运动对卵巢切除所致骨质疏松症大鼠的保护机制。方法 50只雌性SD大鼠,随机分为假手术组(S,15)、造模组(Z,35)。假手术组仅切除卵巢周围的少量脂肪,造模组切除双侧卵巢。术后10周采用苏木精-伊红染色法验证造模。将成功造模的大鼠随机分为模型组(M)、戊酸雌二醇阳性对照组(P)和跑台运动组(E),每组各10只。P组以0.184 mg/(kg·d)的标准灌服戊酸雌二醇混悬液,其他组灌服等剂量生理盐水,每日一次,E组同时进行每周5 d,每次30 min的中等强度跑台训练,干预12周后取材,检测血清抗酒石酸酸性磷酸酶(TRACP)、Ⅰ型前胶原N端肽(PINP)、Ⅰ型胶原交联C-末端肽(CTX)和骨钙素(OC)的水平,Micro-CT检测骨密度,强度仪上检测骨力学性能,Western blot检测股骨中Shh、Ptc、Smo及Gli蛋白表达水平。结果 与S组相比,M组骨形成代谢指标、骨密度值、骨力学性能和股骨Shh、Ptc、Smo及Gli蛋白表达均下降(P<0.01);与M组相比,P组和E组骨形成代谢指标、骨密度值、骨力学性能和股骨Shh、Ptc、Smo及Gli蛋白表达均上升(P<0.01);与P组相比,E组股骨Shh、Ptc、Smo及Gli蛋白表达升高(P<0.05)。结论 12周跑台运动可通过上调股骨Shh、Ptc、Smo及Gli的表达介导对去卵巢大鼠骨代谢的调节,改善骨质疏松症。
英文摘要:
      Objective To explore the protective mechanism of treadmill exercise on ovariectomized osteoporosis rats through Hedgehog-Gli signaling pathway. Methods 50 female SD rats were randomly divided into sham operation group (S, 15) and model group (Z, 35). The sham group removed only a small amount of fat around the ovaries, while the model group removed both ovaries. The model was verified by hematoxylin-eosin staining 10 weeks after operation. The successful rats were randomly divided into model group (M), positive control group (P) and treadmill exercise group (E), with 10 rats in each group. Group P was given 0.184 mg/(kg d) estradiol valerate suspension, while the other groups were given the same dose of normal saline once a day. Group E was also given moderate-intensity treadmill training for 30min minutes, five days a week. After 12 weeks of intervention, blood samples were collected, and the serum levels of procollagen type I N-terminal peptide (PINP), tartrate-resistant acid phosphatase (TRACP), collagen type I cross-linked C- terminal peptide (CTX) and osteocalcin (OC) were detected. Bone mineral density was detected by Micro-CT, bone mechanical properties were detected by material machine, and the expression of Shh, Ptc, Smo and Gli proteins in femur were detected by Western blot. Results Compared with S group, the indexes of bone formation and metabolism, bone mineral density, bone mechanical properties and the expressions of Shh, Ptc, Smo and Gli in femur in group M decreased(P<0.01). Compared with M group, the indexes of bone formation and metabolism, bone mineral density, bone mechanical properties and the expressions of Shh, Ptc, Smo and Gli in f femur in group M decreased(P<0.01). Compared with P group, the expressions of Shh, Ptc, Smo and Gli in femur in group E were higher (P<0.05). Conclusion12 weeks treadmill exercise may mediate the regulation of bone metabolism in ovariectomized rats by up-regulating the expressions of Shh, Ptc, Smo and Gli, and improve osteoporosis.
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