中国骨质疏松杂志

槐黄烷酮G抑制破骨细胞分化和减少骨丢失的机制研究

Sophoraflavanone G inhibits RANKL-induced osteoclastogenesis and alleviates bone loss

DOI：10.3969/j.issn.1006-7108.2024.07.006

英文关键词:osteoporosis osteoclasts Alt Rhizoma drynariae sophoraflavanone G

基金项目:江西中医药管理局科技计划项目（2022A240）；广州中医药大学大学生创新创业训练计划项目（202210572184）

作者	单位
王民政1 李泳渝2 刘为雯2 张诗茵2 梁梓炫2 陈思敏2 郑泉鑫3 黄立宇3 钟露斌4 江政晃5 汤凯2，6*	1.江西中医药大学附属医院,江西南昌 330006 2.广州中医药大学,广东广州 510405 3.广州市正骨医院,广东广州 510055 4.广州粤海医院,广东广州 510430 5.龙川县人民医院,广东河源 517399 6.广州中医药大学第一附属医院,广东广州 510405

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中文摘要:

目的通过体内与体外实验相结合探讨槐黄烷酮G抑制破骨细胞形成和分化，从而缓解骨丢失现象的作用机制研究。方法通过CCK-8和TRAcP染色实验验证槐黄烷酮G是否具有抑制破骨细胞形成的作用；通过分子对接验证槐黄烷酮G与破骨细胞分化相关的特异性蛋白结合的亲和力大小，借助RT-PCR和Western blot实验探讨槐黄烷酮G抑制破骨细胞形成的作用机制；最后通过体内实验进一步探讨，以验证体外实验的准确性和可靠性。结果槐黄烷酮G能够在安全浓度下抑制破骨细胞形成和分化，同时也与破骨细胞相关的特异性蛋白如NFATc1、CTSK、c-Fos、MMP9具有较高的亲和力，并能够降低上述基因和蛋白的相对表达水平，槐黄烷酮G也能够在15和30 min处有效降低MAPK信号通路中的ERK和JNK的磷酸化表达水平，体内实验证明槐黄烷酮G能够起到缓解骨丢失，改善骨小梁相关参数的作用。结论槐黄烷酮G具有抑制破骨细胞形成和分化，缓解骨量减少的作用，从而起到抗骨质疏松症的作用。

英文摘要:

Objective To investigate the mechanism of Sophoraflavanone G in inhibiting osteoclast formation and differentiation, thereby alleviating bone loss, through a combination of in vivo and in vitro experiments. Methods The inhibitory effect of Sophoraflavanone G on osteoclast formation was verified with CCK-8 and TRAcP staining experiments. The affinity of Sophoraflavanone G binding to specific proteins related to osteoclast differentiation was validated with molecular docking. The mechanism of Sophoraflavanone G inhibition of osteoclast formation was explored using RT-PCR and Western blotting experiments. Finally, in vivo experiments were conducted to verify the accuracy and reliability of the in vitro experiments. Results Sophoraflavanone G inhibited the formation and differentiation of osteoclasts at safe concentrations. It had a high affinity with osteoclast-related specific proteins such as NFATc1, CTSK, c-Fos, and MMP9. It reduced the relative expression levels of these genes and proteins. Sophoraflavanone G effectively reduced the phosphorylation expression levels of ERK and JNK in the MAPK signaling pathway at 15 and 30 minutes. In vivo experiments showed that Sophoraflavanone G alleviated bone loss and improved trabecular related parameters. Conclusion Sophoraflavanone G has the ability to inhibit the formation and differentiation of osteoclasts, to alleviate bone loss, thereby playing a role in combating osteoporosis.

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