| Objective Mendelian randomized analysis (MR) was used to explore the causal relationship between glutamine (Gln) and bone mineral density (BMD), bone turnover markers, and Wnt-7a protein. Methods Using Genome-wide association studies (GWAS) data, Gln was selected as the exposure factor. Outcome variables included femoral neck bone mineral density (FN-BMD), forearm bone mineral density (FA-BMD), lumbar bone mineral density (LS-BMD), total body bone mineral density (TB-BMD), and bone turnover indicators, including serum 25-hydroxyvitamin D levels (25(OH)D), parathyroid hormone (PTH), osteopontin (OPG), osteocalcin (BGP), and Wnt signaling family member WNT-7A. Inverse variance weighting method (IVW), MR-Egger regression method, weighted median method, simple mode method and weighted mode method were used. The heterogeneity test, sensitivity analysis, and multieffect analysis were performed. Results MR results showed that there was statistical difference between Gln and Wnt-7a protein, and the relationship between them was positive. There was no significant difference between Gln and FN-BMD, FA-BMD, LS-BMD, TB-BMD, 25(OH)D, PTH, OPG, and BGP. Heterogeneity tests in this study were all normal. The sensitivity analysis results were robust, and no pleiotropy was found. Conclusion In this study, a causal relationship between Gln levels and Wnt-7a was established. The results of this study provide a theoretical basis for the role of Gln in maintaining bone homeostasis and in participating in osteogenic differentiation as well as the prevention and treatment of osteoporosis, suggesting that Gln intake may have potential clinical significance in the prevention and treatment of osteoporosis. |