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| 补益牛膝丸治疗骨质疏松症的研究 |
| Study on tonic radix achyranthis bidentatae pill in the treatment of osteoporosis |
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| DOI：10.3969/j.issn.1006-7108.2025.01.003 |
| 中文关键词: 补益牛膝丸 骨质疏松 “肾主骨”理论 骨形成 协调性 |
| 英文关键词:tonic radix achyranthis bidentatae osteoporosis kidney mastering bone theory bone formation coordination |
| 基金项目:浙江省中医药科技计划项目（2020ZA001，2023ZF097，2023ZL136） |
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| 中文摘要: |
| 目的 探究“肾主骨”理论指导下补益牛膝丸对骨质疏松症模型小鼠的治疗作用。方法 雌性C57BL/6J小鼠15只，随机均分为假手术组、模型组和补益牛膝丸组，模型组和补益牛膝丸组切除双侧卵巢造骨质疏松症模型，补益牛膝丸组小鼠每天进行中药灌胃治疗，其余两组小鼠灌胃等量生理盐水。12周后各组小鼠称重并采集步态数据。取小鼠股骨和胫骨，胫骨进行三点弯曲测试，股骨远端经Micro-CT扫描后观察微结构改变，阿利新蓝/苏木素（alcian blue/haematoxylin and eosin，ABH）染色观察骨小梁数量和形态，免疫组化检测骨碱性磷酸酶(bone alkaline phosphatase，BALP)的表达情况。结果 与模型组相比，补益牛膝丸组小鼠体重下降(P＜0.05)，运动协调性改善，胫骨的弹性模量及承受的最大载荷均升高（P＜0.01，P＜0.001），股骨远端骨密度(bone mineral density，BMD)、骨体积分数(bone volume fraction，BV/TV)、骨小梁厚度(trabecular thickness，Tb.Th)、骨小梁数量(trabecular number，Tb.N)均明显升高（P均＜0.001），骨小梁分离度(trabecular separation，Tb.Sp)下降（P＜0.05），髓腔内骨小梁增加而变致密。ABH染色后补益牛膝丸组股骨髓腔中骨小梁面积大于模型组（P＜0.001）。免疫组化显示补益牛膝丸组BALP阳性表达率高于模型组（P＜0.05）。结论 补益牛膝丸能明显提升骨质疏松症模型小鼠的骨密度，提高小鼠的运动协调性，降低骨质疏松性骨折的风险。 |
| 英文摘要: |
| Objective To explore the therapeutic effect and its potential mechanism of tonic radix achyranthis bidentatae pill on mice with ovariectomized postmenopausal osteoporosis. Methods Fifteen female C57BL/6J mice were randomly divided into sham operation group, model group and tonic radix achyranthis bidentatae group, with 5 mice in each group. Mice in the model group and tonic radix achyranthis bidentatae group underwent bilateral ovariectomy to create osteoporosis model. The mice in tonic radix achyranthis bidentatae group received traditional Chinese medicine every day. Mice in the other two groups received the same amount of normal saline intragastriction. After 12 weeks, mice in each group were weighed and gait data were collected. After collecting the femurs and tibia of mice, the tibia was subjected to three-point bending test. The microstructure of the distal femur was observed with micro-CT scan. ABH staining was used to observe the number and morphology of the bone trabeculae. The expression of BALP in the bone was detected using immunohistochemistry. Results Compared to those in model group, the weight of mice in tonic radix achyranthis bidentatae group decreased (P<0.05), and the motor coordination improved. Elastic modulus and maximum load of the tibia in tonic radix achyranthis bidentatae group increased (P<0.01, P<0.001). Bone mineral density, bone volume fraction, trabecular thickness, and trabecular number of the distal femur increased significantly (all P<0.001), while trabecular separation decreased (P<0.05). The bone trabeculae in the distal medullary cavity of the femur increased and dense. After ABH staining, the trabecular area of the bone marrow cavity in tonic radix achyranthis bidentatae group was larger than that in model group (P<0.001). The immunohistochemistry results showed that the BALP positive expression rate in tonic radix achyranthis bidentatae group was higher than that in model group (P<0.05). Conclusion Tonic radix achyranthis bidentatae significantly increases bone mineral density of mice and treats osteoporosis by tonifying kidney. It improves the motor coordination in mice and reduces the risk of osteoporotic fractures. |
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