中国骨质疏松杂志

松脂醇二葡萄糖苷提高生长期小鼠骨强度的研究

Research on the enhancement of bone strength in growing mice with pinoresinol diglucoside

DOI：10.3969/j.issn.1006-7108.2025.01.006

英文关键词:pinoresinol diglucoside bone mineral density bone microstructure bone strength osteoporosis

基金项目:甘肃省自然科学基金（22JR11RA013）；兰州市科技计划项目（2013-1-8）；中国人民解放军联勤保障部队第九四〇医院青年培育项目（2021yxky053）；中国人民解放军联勤保障部队第九四〇医院实验室培育项目（2021yxky081）

作者	单位
李亮1，2 唐汉琴1，2 马智慧1，2 轩莹莹1，2 杨玉田1，2 高玉海1，2 申栋帅1，2 陈克明1，2*	1.中国人民解放军联勤保障部队第九四〇医院基础医学实验室，甘肃兰州 730050 2.甘肃省干细胞与基因药物重点实验室，甘肃兰州 730050

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中文摘要:

目的探究松脂醇二葡萄糖苷（pinoresinol diglucoside，PDG）对生长期小鼠骨强度的影响，以预防骨质疏松症的发生。方法选取1月龄的C57雌性小鼠，随机分为对照组（Control，等体积的蒸馏水）、PDG-L组[PDG，36 mg/(kg?d)]和PDG-H组[PDG，72 mg/(kg?d)]，每组15只，6周后处死所有动物。进行Micro-CT成像分析、生物力学检测、双荧光标记分析、血清生化指标检测和骨组织相关蛋白表达分析等。结果 PDG给药对小鼠主要脏器未发生明显病理改变；与Control组相比， PDG-L组胫骨的Tb.BMD显著增加（P＜0.01），Tb.N、Tb.Th、Tb.BV/TV明显增加（P＜0.05），Tb.Sp显著降低（P＜0.01），PDG-H组Tb.BMD明显增加（P＜0.05）；PDG-L组股骨的最大载荷和弯曲模量显著提高（P＜0.01），弯曲强度明显高于Control组（P＜0.05），PDG-H组最大载荷和弹性模量也高于Control组（P＜0.05）；PDG各组胫骨皮质骨荧光间距显著增加（P＜0.01），骨形成速率提高，骨矿化沉积速率加快；PDG-L组OPG水平相比于Control组显著提高（P＜0.01），RANKL水平显著降低（P＜0.01）；PDG各组成骨相关蛋白BMP2、OSX和Runx2的表达量显著上调（P＜0.01），骨调节蛋白OPG显著提高（P＜0.01），RANKL显著降低（P＜0.05）。结论 PDG能有效提高骨密度，改善骨微结构，提高骨质量，促进骨形成及抑制骨吸收，提高生长期小鼠的骨强度。

英文摘要:

Objective To investigate the effects of pinoresinol diglucoside (PDG) on bone strength in growing mice, aiming to prevent the development of osteoporosis. Methods One-month-old female C57 mice were randomly divided into three groups: Control (equal volume of distilled water), PDG-L (PDG, 36 mg·kg-1·d-1), and PDG-H (PDG, 72 mg·kg-1·d-1), with 15 animals in each group. All animals were sacrificed after 6 weeks. Micro-CT imaging analysis, biomechanical testing, dual fluorescence labeling analysis, serum biochemical markers detection, and bone tissue-related protein expression analysis were conducted. Results PDG administration did not induce significant pathological changes in major organs of the mice. Compared to the Control group, the PDG-L group showed a significant increase in tibial Tb.BMD (P<0.01), with notable increases in Tb.N, Tb.Th, and Tb.BV/TV (P<0.05), and a significant reduction in Tb.Sp (P<0.01). PDG-H group also showed a significant increase in Tb.BMD (P<0.05). The PDG-L group demonstrated significantly higher maximum load and bending modulus of the femur (P<0.01), with a significantly higher bending strength compared to the Control group (P<0.05). The PDG-H group also showed higher maximum load and elastic modulus compared to the Control group (P<0.05). In both PDG groups, tibial cortical bone fluorescence spacing significantly increased (P<0.01), bone formation rate was elevated, and bone mineralization deposition rate accelerated. PDG-L group showed a significant increase in OPG levels compared to the Control group (P<0.01), and a significant decrease in RANKL levels (P<0.01). The expression levels of bone-related proteins BMP2, OSX, and Runx2 were significantly up-regulated in both PDG groups (P<0.01), as well as a significant increase in bone regulatory protein OPG (P<0.01) and a significant decrease in RANKL (P<0.05). Conclusion PDG effectively enhances bone mineral density, optimizes bone microstructure, improves bone quality, promotes bone formation, and inhibits bone resorption, thereby increases bone strength in growing mice.

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