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| 长春市汉族人群维生素D受体基因多态性、TGF-β、TRACP与腰椎骨密度的相关性 |
| Study on the correlation between vitamin D receptor gene polymorphism, TGF-β, TRACP and lumbar bone density in the Han population of Changchun city |
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| DOI：10.3969/j.issn.1006-7108.2025.04.004 |
| 中文关键词: 维生素D 受体基因 BsmⅠ FokⅠ 转化生长因子-β 抗酒石酸酸性磷酸酶 腰椎 骨密度 |
| 英文关键词:Vitamin D receptor gene BsmⅠ FokⅠ transforming growth factor-β tartrate resistant acid phosphatase lumbar spine bone mineral density |
| 基金项目:吉林省卫生健康技术创新项目（2019J092） |
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| 摘要点击次数: 1 |
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| 中文摘要: |
| 目的 研究长春市35～79岁汉族人群维生素D受体基因BsmⅠ、FokⅠ位点多态性的分布特征及其与腰椎骨密度（bone mineral density，BMD）的相关性，分析不同基因型对骨代谢的调节与影响，同时研究血清TGF-β、TRACP水平与腰椎BMD的相关性，为骨质疏松预防、早期诊断和治疗提供分子生物学依据。方法 应用Hologic Discovery WA型骨密度仪检测腰椎正位（L1-L4）BMD；采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析BsmⅠ和FokⅠ位点多态性；采用酶联免疫吸附分析法检测血清TGF-β、TRACP水平应用；采用SPSS 23.0软件进行统计分析。结果 在500名汉族人群中，BsmⅠ位点基因型以bb型为主，占80.2%，Bb型占15.2%，BB型占4.6%；FokⅠ位点ff基因型占18.6%，Ff型占45.8%，FF型占35.6%。BsmⅠ位点各基因型骨质疏松组与非骨质疏松组分布特征组间差异无统计学意义（P＞0.05）；FokⅠ位点ff基因型骨质疏松组所占比例高于非骨质疏松组，差异有统计学意义（P＜0.05）。BsmⅠ位点bb基因型男性、女性BMD值均低于Bb型和BB型，但差异无统计学意义（P＞0.05）； FokⅠ位点ff基因型男性、女性BMD值均低于Ff型和FF型，差异有统计学意义（P＜0.05）。血清TGF-β水平骨质疏松组显著低于非骨质疏松组，而TRACP水平骨质疏松组高于非骨质疏松组，组间差异有统计学意义（P＜0.05）。结论 本研究500名汉族人群中，BsmⅠ位点以bb基因型为主，占80.2%，各基因型骨密度值组间差异无统计学意义；FokⅠ位点ff型BMD值低于Ff型和FF型，骨质疏松组ff基因型所占比例高于非骨质疏松组，差异有统计学意义，提示ff 基因型可能是骨质疏松发生的危险因素；骨质疏松组血清TGF-β水平显著低于非骨质疏松组，而TRACP水平高于非骨质疏松组，差异有统计学意义，表明TGF-β、TRACP是评价骨代谢状态的良好指标。 |
| 英文摘要: |
| Objective Study the distribution characteristics of vitamin D receptor gene Bsm Ⅰ and Fok Ⅰ polymorphism in the Han population aged 35-79 in Changchun city and their correlation with lumbar bone mineral density (BMD), analyzing the regulation and influence of different genotypes on bone metabolism. Simultaneously investigate the correlation between serum TGF - β, TRACP levels and lumbar BMD. In order to provide molecular biology basis for the prevention, early diagnosis and treatment of osteoporosis. Methods Hologic Discovery WA bone densitometer was used to detect lumbar spine anteroposterior (L1-L4) BMD; polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was used to analyze the polymorphism of Bsm Ⅰ and Fok Ⅰ; Application of enzyme-linked immunosorbent assay to detect serum TGF - β and TRACP levels; Statistical analysis was conducted using SPSS 23.0 software. Results Among the 500 Han population, the genotype of Bsm Ⅰ was mainly bb type, accounting for 80.2%, Bb type accounted for 15.2%, and BB type accounted for 4.6%; ff genotype of Fok Ⅰ accounted for 18.6%, Ff genotype accounted for 45.8%, and FF genotype accounted for 35.6%. There was no statistically significant difference (P>0.05) in the distribution characteristics of each genotype of Bsm Ⅰ between the osteoporosis group and the non osteoporosis group; The proportion of Fok Ⅰff type in osteoporosis group was higher than that in non osteoporosis group, and the difference is statistically significant (P<0.05). The BMD values of both males and females with the bb genotype of Bsm Ⅰwere lower than those of the Bb and BB genotypes, but there was no statistically significant difference (P>0.05); The BMD values of both males and females with the Fok Ⅰff genotype were lower than those of the Ff and FF genotypes, and the difference was statistically significant (P<0.05). The serum TGF-β level in the osteoporosis group was significantly lower than that in the non osteoporosis group, while the TRACP level was higher than that in the non osteoporosis group, and the difference between the groups was statistically significant (P<0.05). Conclusion Among the 500 participants in this study, the Bsm Ⅰis mainly bb genotype, accounting for 80.2%, and there was no statistically significant difference in bone density values between different genotypes; The BMD values of the Fok Ⅰ site ff type were lower than those of the Ff type and FF type, and the proportion of ff type in the osteoporosis group was higher than that in the non osteoporosis group, and the difference is statistically significant with statistical significance, suggesting that ff genotype may be a risk factor for osteoporosis; The serum TGF-β level in the osteoporosis group was lower than that in the non osteoporosis group, while the TRACP level was significantly higher than that in the non osteoporosis group, and the difference was statistically significant, indicating that TGF-β and TRACP are good indicators for evaluating bone metabolism status. |
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