中国骨质疏松杂志

煨肾汤调控TGF-β/Smad2与MAPK通路对骨质疏松骨保护的研究

Study on the bone protection of osteoporosis by the regulation of TGF-β/Smad2 and MAPK pathways

DOI：10.3969/j.issn.1006-7108.2025.06.004

中文关键词: 煨肾汤老年性骨质疏松 TGF-β/Smad2 SAMP6小鼠

英文关键词:Weishen decoction senile osteoporosis TGF-β/Smad2 SAMP6

基金项目:徐州市科技重点研发项目(KC22176)；江苏省中医科技发展面上项目(MS2023078、MS2023077)

作者	单位
张鹏1 高莎1 魏浩洋1 王璐璐1 张洋1,2*	1.徐州市中心医院，江苏徐州 221009 2.徐州医科大学徐州临床学院，江苏徐州 221009

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中文摘要:

目的探讨煨肾汤对快速老化骨质疏松小鼠(SAMP6)的骨保护作用及机制。方法 30只SAMP6小鼠随机分为SOP模型组、碳酸钙+骨化醇组、煨肾汤组，10只正常老化SAMR1小鼠作为正常对照组。煨肾汤组以煨肾汤浓煎液灌胃，碳酸钙+骨化醇组以碳酸钙D3、阿法骨化醇混悬液灌胃，SOP模型组和正常对照组予以同体积生理盐水。治疗3个月，取血清及股骨。检测小鼠骨密度、骨组织病理、骨代谢指标，TGF-β、Smad2通路蛋白，MAPK通路p-JNK、p-ERK、p38、NF-κB p65、p50蛋白。结果与正常对照组相比，SOP模型组小鼠骨质紊乱、骨小梁减少，骨密度、钙(Ca)、磷(P)、骨碱性磷酸酶(BALP)、抗酒石酸酸性磷酸酶(TRACP)、I型原胶原N-端前肽(P1NP)、信号通路TGF-β、Smad2、p-JNK、p-ERK、p38、p65、p50蛋白较正常对照组差异均有统计学意义(P＜0.01)。与SOP模型组相比，煨肾汤组骨质结构改善，骨密度和骨代谢指标Ca、BALP、P1NP显著升高(P＜0.01)，TGF-β、Smad2、p-JNK、p-ERK、p38蛋白显著升高(P＜0.01)，P、TRACP显著降低(P＜0.01)，p65、p50蛋白显著降低(P＜0.01)。与碳酸钙+骨化醇组相比，煨肾汤组P1NP显著升高(P＜0.01)，TRACP降低(P＜0.01)，TGF-β、Smad2、p-JNK、p-ERK、p38蛋白显著升高(P＜0.01)，p65、p50显著降低(P＜0.01)。结论煨肾汤可通过调节TGF-β/Smad2与MAPK通路改善骨细胞活性，调节骨代谢，促进骨形成，改善骨密度。

英文摘要:

Objective To study the effect and mechanism of Weishen decoction on bone protection in senescence-accelerated mouse prone 6 (SAMP6). Methods Thirty SAMP6 mice were randomly divided into SOP model group, calcium carbonate + calcitol group and Weishen decoction group. Ten SAMR1 mice aged normally were used as normal control group. Weishen decoction group was given Weishen thick decoction , calcium carbonate + calcitol group was given calcium carbonate D3 and alpha calcitol suspension, SOP model group and normal control group were given the same volume of normal saline. After 3 months of treatment, serum and femur were taken. Bone mineral density, bone histopathology, bone metabolism, TGF-β, Smad2 pathway protein, MAPK pathway p-JNK, p-ERK, p38, NF-κB p65, p50 proteins were detected. Result Compared with the normal control group, the bone trabeculae of the mice with bone disorder in SOP model group were reduced. There were significant differences in bone mineral density, calcium (Ca), phosphorus (P), bone alkaline phosphatase (BALP), tartrate-resistant acid phosphatase (TRACP), procollagen type I N-terminal propeptide (P1NP), signaling pathway TGF-β, Smad2, p-JNK, p-ERK, p38, p65 and p50 proteins compared with normal control group (P＜0.01). Compared with SOP model group, the bone structure of Weishen decoction group was improved, bone mineral density and bone metabolism indexes Ca, BALP and P1NP were significantly increased (P＜0.01), and the protein expressions of TGF-β, Smad2, p-JNK, p-ERK and p38 were significantly increased (P＜0.01). P and TRACP were decreased (P＜0.01), p65 and p50 proteins were decreased (P＜0.01). Compared with calcium carbonate + calcitol group, P1NP was significantly increased (P＜0.01), TRACP was decreased (P＜0.01), TGF-β, Smad2, P-JNK, P-ERK and p38 proteins were significantly increased (P＜0.01), and p65 and p50 proteins were decreased (P < 0.01) in Weishen decoction group. Conclusion Weishen decoction can improve bone cell activity, regulate bone metabolism, promote bone formation and increase bone density by regulating TGF-β/Smad2 and MAPK pathways.

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