| Objective To study the serum levels of FlOP, β-crosslaps, CatheK and their correlation with lumbar BMD in 500 individuals aged 20-80 years, and to provide molecular biology basis for the diagnosis, differential diagnosis, and efficacy evaluation of metabolic bone diseases, osteoporosis, bone and immune diseases. Methods Fluorescence micropore technology was used to detect serum FlOP levels, and enzyme-linked immunosorbent assay (ELISA) was used to measure serum β-crosslaps and Cathe K marker levels. Dual energy X-ray absorptiometry was used to detect lumbar spine BMD (L1-L4), and SPSS 23.0 software was used for statistical analysis. Results The results of lumbar spine anteroposterior (L1-L4) BMD testing showed that after the age of 40, BMD showed a decreasing trend with age, and there was a statistically significant difference between the groups (P<0.05); The detection results of serum FlOP-320, FlOP-360, and FlOP-400 in different age groups showed that the detection values of FlOP-320 and FlOP-400 increased with age, but there was no statistical difference between the age groups (P>0.05). The detection value of FlOP-360 gradually increased, and there was a statistical difference between the age group after 60 years old and other age groups (P<0.05); The detection values of serum β - crosslaps and Cathe K in different age groups gradually increase with age, and the detection values significantly increase after the age of 50. Compared with other age groups, the difference is statistically significant (P<0.05); The correlation analysis between the detection values of FlOP, β -crosslaps, Cathe K and lumbar spine BMD (L1-4) showed that there was no correlation between the detection values of FlOP-320 and FlOP-400 and lumbar spine BMD. There is a negative correlation between serum FlOP-360 and lumbar spine BMD, with statistical significance (P<0.05). β-crosslaps, Cathe K, and lumbar spine BMD were negatively correlated with statistical significance (P<0.05). Conclusion As age increases, there is a statistically significant difference in the detection value of FlOP-360 between the age group after 60 years old and other age groups, which is negatively correlated with lumbar spine BMD. The detection values of serum β- crosslaps and Cathe K significantly increase after the age of 50, and are negatively correlated with lumbar spine BMD. The levels of serum FlOP-360, β-crosslaps, and Cathe K are negatively correlated with lumbar spine BMD and are good indicators for evaluating bone metabolism status. They have important clinical significance for the diagnosis, differential diagnosis, and efficacy evaluation of metabolic bone diseases, osteoporosis, bone and immune disorders, and other diseases. |