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| 振动疗法介导Piezo1骨质疏松大鼠H型血管生成 |
| Vibration therapy mediates H-type angiogenesis in Piezo1 osteoporotic rats |
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| DOI：10.3969/j.issn.1006-7108.2025.08.001 |
| 中文关键词: 全身振动疗法 激素性骨质疏松 Piezo1 H型血管 |
| 英文关键词:whole body vibration therapy glucocorticoid-induced osteoporosis piezo1 type H blood vessels |
| 基金项目:国家自然科学基金面上项目（82274544）；广东省基础与应用研究基金项目（2023A1515010551）；毕节市科学技术局“揭榜挂帅”项目[毕科合重大专项(2022)1号]；中国博士后科学基金面上项目（2024M750819）；广州中医药大学青年拔尖人才（团队）揭榜挂帅项目；中医证候全国重点实验室研究生项目（SKLKY2024A0003） |
| 作者 | 单位 | | 廖家如1,2 杨晓强1,2,3 侯文渊1,2 田佳庆2 林锟1,2 张俊娇1,2 韩龙飞1,2 陆舜1,2 杨帆4,5 何敏聪4,5,6 陈冠林4 魏秋实4,5* | 1 广州中医药大学第三临床医学院，广东 广州 510006
2广州中医药大学，广东 广州 510006
3 中医证候全国重点实验室/骨科，广东 广州 510357
4 广州中医药大学第三附属医院，广东 广州 510357
5 广东省中医骨伤研究院，广东 广州 510375
6 河南省洛阳正骨医院(河南省骨科医院)博士后工作站，河南 洛阳 471000 |
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| 中文摘要: |
| 目的 观察全身振动疗法对激素性骨质疏松大鼠骨组织Piezo1表达和H型血管形成的影响，探讨全身振动疗法防治骨质疏松症的作用机制。方法 构建激素性骨质疏松大鼠模型，分为空白组、激素组、WBVT组、Yoda1组、WBVT+GxMTs4组。经治疗后， 通过Micro-CT分析骨组织形态变化和骨量变化；HE染色观察骨组织病理学改变；免疫组织化学检测Piezo1表达，成骨相关蛋白BMP2、RUNX2表达，成血管相关蛋白HIF-α、VEGFA表达；免疫荧光检测H型血管相关蛋白EMCN、CD31表达。结果 与空白组比，激素组的骨密度下降，骨小梁间隔增大、不连续，胫骨中Piezo1、BMP2、RUNX2、HIF-1α、VEGFA、EMCN、CD31表达下降；与激素组比，WBVT组与Yoda1组的骨密度上升，骨小梁间隔减小、较连续，胫骨中Piezo1、BMP2、RUNX2、HIF-1α、VEGFA、EMCN、CD31表达上升；与WBVT组比，WBVT+GxMTs4组的骨密度下降，骨小梁间隔增大、不连续，胫骨中Piezo1、BMP2、RUNX2、HIF-1α、VEGFA、EMCN、CD31表达下降。结论 全身振动疗法在调节骨骼代谢方面展现出显著效果，具备抗骨质疏松潜能。其作用机制可能是通过Piezo1信号通路促进H型血管形成来实现的。 |
| 英文摘要: |
| Objective To observe the effects of whole-body vibration therapy on Piezo1 expression in bone tissue and Type H blood vessel formation in glucocorticoid-induced osteoporotic rats, and to explore the mechanism of action of whole-body vibration therapy in preventing and treating osteoporosis. Methods A glucocorticoid-induced osteoporotic rat model was established and divided into blank group, hormone group, WBVT group, Yoda1 group, and WBVT+GxMTs4 group. After treatment, Micro-CT was used to analyze changes in bone tissue morphology and bone mass. HE staining was performed to observe histopathological changes in bone tissue. Immunohistochemistry was used to detect Piezo1 expression, as well as expression of osteogenic proteins BMP2 and RUNX2, and angiogenic proteins HIF-α and VEGFA. Immunofluorescence was employed to detect Type H blood vessel-related proteins EMCN and CD31 expression. Results Compared with the blank group, the hormone group showed decreased bone density, increased and discontinuous trabecular spacing in the tibia, and decreased expression of Piezo1, BMP2, RUNX2, HIF-1α, VEGFA, EMCN, and CD31. Compared with the hormone group, the vibration and Yoda1 groups exhibited increased bone density, decreased and more continuous trabecular spacing in the tibia, and increased expression of Piezo1, BMP2, RUNX2, HIF-1α, VEGFA, EMCN, and CD31. Compared with the vibration group, the WBVT+GxMTs4 group showed decreased bone density, increased and discontinuous trabecular spacing in the tibia, and decreased expression of Piezo1, BMP2, RUNX2, HIF-1α, VEGFA, EMCN, and CD31. Conclusion Whole-body vibration therapy demonstrated significant effects in regulating bone metabolism and has potential as an anti-osteoporosis treatment. Its mechanism of action may involve promoting Type H blood vessel formation through the Piezo1 signaling pathway. |
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