| Objective To clarify the effects of blood components of Yougui Wan on the mRNA expressions of Wnt/β-catenin and OPG/RANKL/RANK pathway related factors in MC3T3-E1 and RAW264.7 cells. Methods MC3T3-E1 and RAW264.7 cells were cultured for osteogenic and osteoclast induction, respectively. Monomer intervention solution containing blood components of Yougui Wan was prepared for cell intervention. RT qPCR was used to detect the expressions of Lrp6, Lrp5, β - catenin, Tcf7, Fzd2, and OPG in MC3T3-E1 cells and Traf6, Nfatc1, Fos, Mmp9, Ctsk, and Car2 mRNA in RAW264.7 cells. Results The staining showed that MC3T3-E1 cells were blue-purple with orange-red calcium deposits, and RAW264.7 cells were burgundy. Compared to that in the blank control group, aconitine significantly inhibited the mRNA expressions of Traf6, Fos, and Mmp9 (P<0.05). Subaconitine and neoaconitine significantly inhibited the mRNA expression of Fos (P<0.05). Benzoyl aconitine significantly up-regulated the mRNA expressions of Lrp6, β-catenin, Tcf7, Fzd2, and OPG (P<0.05), and significantly down-regulated the mRNA expressions of Traf6, Fos, and Mmp9 (P<0.05). Benzoyl aconitine promoted mRNA expressions of Lrp5, β-catenin, Tcf7, Fzd2, and OPG (P<0.05). Benzoyl neoaconitine promoted mRNA expressions of Lrp6, Lrp5, Tcf7, and OPG (P<0.05), and inhibited the expressions of Traf6, Fos, Mmp9, and Car2 (P<0.05). Pineolin glucoside promoted the mRNA expressions of Lrp5, β-catenin, Tcf7, and OPG (P<0.05), and significantly down-regulated the mRNA expressions of Traf6, Ctsk, and Car2 (P<0.05). Cinnamic acid significantly up-regulated the mRNA expressions of Lrp5, Tcf7, Fzd2, and OPG (P<0.05), and significantly inhibited the mRNA expressions of Nfatc1 and Fos (P<0.05). Conclusion The blood components of Yougui Wan regulates the gene expressions of Wnt/β-catenin and OPG/RANKL/RANK signaling pathway related factors within a certain concentration range, affects bone formation and resorption, and plays a role in the treatment of osteoporosis. |